氰戊菊酯对黄胫小车蝗解毒酶的影响

金永玲; 杨苏宁; 丛斌; 王丽艳

doi:10.11829/j.issn.1001-0629.2015-0107

氰戊菊酯对黄胫小车蝗解毒酶的影响

金永玲^1,2,3,
杨苏宁⁴,
丛斌⁵,
王丽艳⁴

1.
1. 沈阳农业大学植物保护学院,辽宁沈阳 110161
2.
2. 黑龙江八一农垦大学农学院,黑龙江大庆 163319
3.
4.
黑龙江八一农垦大学农学院,黑龙江大庆 163319
5.
沈阳农业大学植物保护学院,辽宁沈阳 110161

基金项目:

国家公益性行业(农业)科研专项(201003079)

黑龙江省寒地作物种质改良与栽培重点实验室支持项目(CGIC201208 )

摘要: 为了确定氰戊菊酯对黄胫小车蝗(Oedaleus infernalis)解毒酶的影响,利用点滴法进行毒力测定的同时,针对不同虫源黄胫小车蝗测定了增效醚(PBO)、磷酸三苯酯(TPP)和顺丁烯二酸二乙酯(DEM)3种酶抑制剂的增效作用和虫体内解毒酶的活力。结果表明,氰戊菊酯对室内虫源的LD50为4.38×10-3 μg·头-1,对黑龙江地区田间虫源HG、LD和ZY 的LD50分别7.30×10-3、7.21×10-3和2.298×10-2 μg·头-1,与室内虫源相比,抗性比分别为1.67、1.65和5.25倍。PBO对室内虫源和田间虫源ZY的增效比分别为2.16、3.91倍,TPP对室内虫源和田间虫源ZY的增效比分别为2.00、2.15倍。田间虫源ZY体内酯酶及多功能氧化酶活性均显著高于室内虫源(P<0.05)。经氰戊菊酯诱导后,室内虫源和田间虫源ZY体内的酯酶上升比值分别为2.25、1.59倍,多功能氧化酶活性分别上升1.21、1.45倍。以上结果表明,田间虫源ZY黄胫小车蝗对氰戊菊酯的敏感性下降,且其体内酯酶、多功能氧化酶活性提高,说明酯酶和多功能氧化酶参与了氰戊菊酯的代谢作用,推断这两种酶是导致黄胫小车蝗氰戊菊酯抗性的原因之一。

关键词:

English

Effects of fenvalerate on detoxifying enzyme activities of Oedaleus infernalis

1.
1. Plant Protection College, Shenyang Agricultural University, Shenyang 110161, China
2.
2. Agricultural College, Heilongjiang Bayi Agricultural University, Daqing 163319, China
3.
4.
Agricultural College, Heilongjiang Bayi Agricultural University, Daqing 163319, China
5.
Plant Protection College, Shenyang Agricultural University, Shenyang 110161, China

Received Date: 2015-02-13
Publish Date: 2015-11-19

Abstract: In order to determine the effects of fenvalerate on detoxification enzymes activities of Oedaleus infernalis, the enzyme inhibitors (piperonyl butoxide, PBO; three phosphoric acid phenyl esterm, TPP; diethylmeteate, DEM) effects synergy with in vivo enzyme activities in insects from different source were measured. The results showed that the LD50 of fenvalerate to in door population source O. infernalis was 4.38×10-3 μg·locust-1, to the field population source HG, LD and ZY in the Heilongjiang were 7.30×10-3 μg·locust-1, 7.21×10-3 μg·locust-1 and 22.98×10-3 μg·locust-1, respectively, which had the resistance ratio of 1.67, 1.65 and 5.25. Both PBO and TPP on indoor and field population source ZY to fenvalerate had synergistic effects, which had the synergistic ratio 2.16 and 3.91, 2.00 and 2.15, respectively. Comparing with field population source ZY, the activities of esterase and multiple function oxidase in the indoor population source significantly increased (P<0.05). After the induction of fenvalerate, the esterase activities rising ratios of field population source ZY and indoor population source were 2.25 and 1.59; and the multiple function oxidase activities rising ratio were 1.21 and 1.45, respectively. These above results showed that the activities of esterase and multiple function oxidase increased with the decreasing of sensitivity of O. infernalis to fenvalerate which suggested that esterase and multiple function oxidase were involved in the metabolism of fenvalerate in oedaleus infernalis, and the two enzymes wereone of the reasons for fenvalerate resistance.

Keywords:

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参考文献(1)

[1]

贺艳萍.中国重要蝗区东亚飞蝗有机磷杀虫剂抗性生化机制研究[D].太原:山西大学博士学位论文,2004,[12] 黄广文,于钊,张蓉,朱猛蒙,魏淑花.宁夏天然草原蝗虫生物多样性及其对生境的指示作用[J],草业科学,2014,31(1):180-186.[13]黄文广,于钊,张蓉,王蕾,张宇,朱猛蒙,魏淑花.盐池县典型草原蝗虫发生与植被群落的关系[J].草业科学.2014,31(2):306-312[15] 孙嵬,张柱亭,类成平,董辉,钱海涛,丛斌.不同地理种群黄胫小车蝗的遗传多样性及遗传分化研究[J].沈阳农业大学学报,2013,44(6)748-753.[22] 张燕宁,何伟志,蒋红云,赵冬雪.防治蝗虫有效药剂的室内筛选[A].植物保护科技创新与发展——中国植物保护学会2008年学术年会论文集[C].重庆:中国植物保护学会,2008:903-905.

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氰戊菊酯对黄胫小车蝗解毒酶的影响

English

Effects of fenvalerate on detoxifying enzyme activities of Oedaleus infernalis

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