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紫花苜蓿病毒病症状类型及病原检测

周其宇, 梁巧兰, 韩亮

周其宇, 梁巧兰, 韩亮. 紫花苜蓿病毒病症状类型及病原检测[J]. 草业科学, 2016, 10(7): 1297-1305. DOI: 10.11829/j.issn.1001-0629.2015-0652
引用本文: 周其宇, 梁巧兰, 韩亮. 紫花苜蓿病毒病症状类型及病原检测[J]. 草业科学, 2016, 10(7): 1297-1305. DOI: 10.11829/j.issn.1001-0629.2015-0652
Qi-yu Zhou, Qiao-lan Liang, Liang Han. Symptoms and pathogen detection of alfalfa virus disease[J]. Pratacultural Science, 2016, 10(7): 1297-1305. DOI: 10.11829/j.issn.1001-0629.2015-0652
Citation: Qi-yu Zhou, Qiao-lan Liang, Liang Han. Symptoms and pathogen detection of alfalfa virus disease[J]. Pratacultural Science, 2016, 10(7): 1297-1305. DOI: 10.11829/j.issn.1001-0629.2015-0652

紫花苜蓿病毒病症状类型及病原检测

基金项目: 

草业生态系统教育部重点实验室开放课题(CYZS-2011006)

甘肃省财政厅项目(甘财教2013(116))

摘要: 本研究为了探明甘肃省兰州市紫花苜蓿(Medicago sativa)侵染病毒病的症状类型、病原种类以及寄主范围,进行了田间调查和室内酶联免疫检测。田间调查确定了苜蓿病毒病在兰州市安宁区和皋兰县以及白银市景泰县均有发生,其中景泰县的发病率和病情指数均最高,分别为43.33%和26.08;共采样90份,检查30份,检查比例为30%。检测到的病原有苜蓿花叶病毒(AMV)、白三叶草花叶病毒(WCMV)、菜豆黄花叶病毒(BYMV)和豇豆花叶病毒(CPMV)4种,检出率分别为100.00%、50.00%、75.00%和75.00%,且存在复合侵染现象,复合侵染率达50.00%;寄主范围测定供试植物共6科17属18种,经测定,AMV可以侵染6科15种植物,WCMV可以侵染4科8种植物,BYMV和CPMV可以侵染3科7种植物,4种病毒可复合侵染6科14种植物。

 

English

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  • 收稿日期:  2015-11-22
  • 发布日期:  2016-07-19

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