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玉米秸秆和白菜尾菜混贮料的乳酸菌多样性及耐高温优良菌株筛选

李志忠, 窦俊伟, 任海伟, 李金平, 孙文斌, 黄娟娟, 李梦玉

李志忠, 窦俊伟, 任海伟, 李金平, 孙文斌, 黄娟娟, 李梦玉. 玉米秸秆和白菜尾菜混贮料的乳酸菌多样性及耐高温优良菌株筛选[J]. 草业科学, 2017, 11(6): 1337-1346. DOI: 10.11829/j.issn.1001-0629.2016-0442
引用本文: 李志忠, 窦俊伟, 任海伟, 李金平, 孙文斌, 黄娟娟, 李梦玉. 玉米秸秆和白菜尾菜混贮料的乳酸菌多样性及耐高温优良菌株筛选[J]. 草业科学, 2017, 11(6): 1337-1346. DOI: 10.11829/j.issn.1001-0629.2016-0442
Zhi-zhong Li, Jun-wei Dou, Hai-wei Ren, Jin-ping Li, Wen-bin Sun, Juan-juan Huang, Meng-yu Li. Lactic acid bacteria diversity analysis and screening of superior thermotolerant strains from corn stover and cabbage waste mixed-silages[J]. Pratacultural Science, 2017, 11(6): 1337-1346. DOI: 10.11829/j.issn.1001-0629.2016-0442
Citation: Zhi-zhong Li, Jun-wei Dou, Hai-wei Ren, Jin-ping Li, Wen-bin Sun, Juan-juan Huang, Meng-yu Li. Lactic acid bacteria diversity analysis and screening of superior thermotolerant strains from corn stover and cabbage waste mixed-silages[J]. Pratacultural Science, 2017, 11(6): 1337-1346. DOI: 10.11829/j.issn.1001-0629.2016-0442

玉米秸秆和白菜尾菜混贮料的乳酸菌多样性及耐高温优良菌株筛选

基金项目: 

国家自然科学基金(51366009、51666010)

国家高技术研究发展计划(863)(2014AA052801)

甘肃省自然科学基金项目(1506RJYA106、1508RJYA097)

兰州市科技计划项目(2014-2-20)

摘要: 通过形态特征、生理生化特性及16S rRNA序列分析方法对玉米(Zea mays)秸秆和白菜(Brassica pekinensis)尾菜混贮料中的乳酸菌多样性进行分析,并以温度和pH为限制因素筛选优良乳酸菌菌株。结果表明,分离得到的12株乳酸菌分属于乳杆菌属(Lactobacillus)和片球菌属(Pediococcus)。其中,1株(LB-1)为植物乳杆菌(Lactobacillus plantarum),6株(LB-2、LB-4、LB-7、LB-8、LB-9和LB-11)为戊糖片球菌(Pediococcus pentosaceus),3株(LB-5、LB-6和LB-12)为短乳杆菌(Lactobacillus brevis),2株(LB-3和LB-10)为类干酪乳杆菌(Lactobacillus paracasei)。菌株LB-3和LB-8表现出优良的耐高温、耐酸碱特性,且具有较强的产乳酸能力,二者可作为青贮饲料的乳酸菌添加剂。

 

English

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文章相关
  • 收稿日期:  2016-08-22
  • 修回日期:  2017-03-03
  • 发布日期:  2017-06-19

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