差异光周期下野牛草相连分株内源激素的日节律

李伟; 钱永强; 韩蕾; 孙振元

doi:10.11829/j.issn.1001-0629.2016-0638

差异光周期下野牛草相连分株内源激素的日节律

李伟^1,2,3,
钱永强⁴,
韩蕾⁴,
孙振元⁴

1.
1. 中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京 100091
2.
2. 青岛农业大学园林与林学院,山东青岛 266109
3.
4.
中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京 100091

基金项目:

国家自然科学基金&#x02014

&#x02014

差异光周期作用下野牛草相连克隆分株间生物节律同步化调控机理(31100505)

摘要: 为了分析野牛草(Buchloe dactyloides)相连克隆分株在差异光周期下内源激素的变化,利用酶联免疫吸附测定法(ELISA)对野牛草相连克隆分株在相反光照条件下的植物内源激素生长素(IAA)、赤霉素(GA)、脱落酸(ABA)、玉米素核苷(ZR)含量进行了测定,探讨植物内源激素在生物节律调控过程中的作用机理。结果表明,野牛草内源激素IAA、GA、ABA及ZR含量在一天内呈现节律性变化规律,同时,在差异光周期下,野牛草相连克隆分株叶片的内源激素水平趋向于同步化,并且倾向于内源生物节律较强的分株。其中,差异光周期处理1周后,在全光照条件下,野牛草克隆分株在断开状态时,叶片中IAA、GA、ABA及ZR含量在24 h内基本呈现相反的变化趋势,而野牛草克隆分株叶片中IAA、GA、ABA及ZR含量在24 h内的变化趋于一致。

关键词:

English

Circadian rhythm variation of endogenous hormones in Buchloe dactyloides connected ramets under differential photoperiod

1.
1. Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, CAF, Beijing 100091, China
2.
2. College of Landscape Architecture and Forestry, Qingdao Agricultural University, Qingdao 266109, China
3.
4.
Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, CAF, Beijing 100091, China

Received Date: 2016-12-26
Publish Date: 2017-09-19

Abstract: In order to understand the endogenous hormonal differences in Buchloe dactyloides connected ramets under different photoperiods, the levels of four endogenous hormones [indole-3-acetic acid (IAA), gibberellin (GA), abscisic acid (ABA) and zeatin (ZR)] in buffalo grass leaves were determined using ELISA. The results indicated that IAA, GA, ABA and ZR levels of buffalo grass reveal rhythmic variations on a daily basis, while the endogenous hormone levels of buffalo grass ramets tend to be synchronized even under different photoperiods. After one week of different photoperiod treatments, under continuous light conditions, IAA, GA, ABA and ZR content in disconnected ramets of buffalo grass leaves basically showed the opposite trend within 24 h, however, IAA, GA, ABA and ZR content in connected ramets of buffalo grass leaves were synchronization trend within 24 h.

Keywords:

HTML全文

参考文献(40)

[1]	Luo F C,Guo Y M,Peng J,Duan X H,Xu W H,He C,Guo F G.Effects of exogenous hormones treatments to release seeds dormancy of Setaria sphacelata cv. Narok.Pratacultural Science,2015,32(3):406-412.(in Chinese)
[1]	Lumsden P J,Millar A J.Biological rhythms and photoperiodism in plants.Quarterly Review of Biology.Oxford,Washington DC:BIOS Scientific Publishers,1998.
[2]	Han Y H,Wang Y R,Tao Q B.Advances of seed hormonal priming.Pratacultural Science,2016,33(12):2494-2502.(in Chinese)
[2]	Young M W,Kay S A.Time zones:A comparative genetics of circadian clocks.Nature Reviews Genetics,2001,2(9):702-705.
[3]	Zhai X M,Zang C X,Wang M,Ren M Y,Guan X.Effect of CO 2 enrichment on hormone content of different types fall dormancy alfalfa.Pratcaultural Science,2016,33(3):442-449.(in Chinese)
[3]	Webb A A R.The physiology of circadian rhythms in plants.New Phytologist,2003,160(2):281-303.
[4]	Dodd A N,Salathia N,Hall A,K#x000e9;vei E,T#x0dbc0;#x0dda9;th R,Nagy F,Hibberd J M,Millar A J,Webb A A.Plant circadian clocks increase photosynthesis,growth,survival and competitive advantage.Science,2005,309:630-633.
[5]	Hotta C T,Gardner M J,Hubbard K E,Baek S J,Dalchau N,Suhita D,Dodd A N,Webb A A.Modulation of environmental responses of plants by circadian clocks.Plant,Cell #x00026; Environment,2007,30(3):333-349.
[6]	Yanovsky M J,Kay S A.Signaling networks in the plant circadian system.Current Opinion in Plant Biology,2001,4(5):429-435.
[7]	Todd P M,Patrice A S,Hannah J Y,Taylor R S,Emily L S,Mark A M,Jos#x000e9; M A,Joseph R E,McClung C R.Enhanced fitness conferred by naturally occurring variation in the circadian clock.Science,2003,302:1049-1053.
[8]	Fedoroff N V.Cross-talk in abscisic acid signaling.Sciences Stke Signal Transduction Knowledge Environment,2002(140):10.
[9]	Srivastava L M.Plant growth and development:Hormones and environment.Plant Growth #x00026; Development Hormones #x00026; Environment,2002,16(S1):68-74.
[10]	罗富成,郭轶敏,彭健,段新慧,许文花,何超,郭凤根.外源激素对纳罗克非洲狗尾草种子休眠的破除效果.草业科学,2015,32(3):406-412.
[11]	韩云华,王彦荣,陶奇波.种子激素引发.草业科学,2016,33(12):2494-2502.
[12]	Mizuno T,Yamashino T.Comparative transcriptome of diurnally oscillating genes and hormone-responsive genes in Arabidopsis thaliana :Insight into circadian clock-controlled daily responses to common ambient stresses in plants.Plant and Cell Physiology,2008,49(3):481-487.
[13]	Robertson F C,Skeffington A W,Gardner M J,Webb A A.Interactions between circadian and hormonal signalling in plants.Plant Molecular Biology,2009,69(4),419-427.
[14]	Jouve L,Gaspar T,Kevers C,Greppin H,Agosti R D.Involvement of indole-3-acetic acid in the circadian growth of the first internode of Arabidopsis.Planta,1999,209(1):136-142.
[15]	Nov#x000e1;kov#x000e1; M,Motyka V,Dobrev P I,Malbeck J,Gaudinov#x000e1; A,Vankov#x000e1; R.Diurnal variation of cytokinin,auxin and abscisic acid levels in tobacco leaves.Journal of Experimental Botany,2005,56:2877-2883.
[16]	Kunihiro A,Yamashino T,Nakamichi N,Niwa Y,Nakanishi H,Mizuno T.Phytochrome-interacting factor 4 and 5 (PIF4 and PIF5) activate the homeobox ATHB2 and auxin-inducible IAA29 genes in the coincidence mechanism underlying photoperiodic control of plant growth of Arabidopsis thaliana .Plant and Cell Physiology,2011,52(8):1315-1329.
[17]	Alabad#x000ed; D,Gil J,Bl#x000e1;zquez M A,Garc#x000ed;a-Mart#x000ed;nez J L.Gibberellins repress photomorphogenesis in darkness.Plant Physiology,2004,134(3):1050-1057.
[18]	Alabad#x000ed; D,Gallego-Bartolom#x000e9; J,Orlando L,Garc#x000ed;a-C#x000e1;rcel L,Rubio V,Mart#x000ed;nez C,Frigerio M,Iglesias-Pedraz J M,Espinosa A,Deng X W,Bl#x000ed;zquez M A.Gibberellins modulate light signaling pathways to prevent Arabidopsis seedling de-etiolation in darkness.Plant Journal for Cell #x00026; Molecular Biology,2008,53(2):324-335.
[19]	Foster K R,Morgan P W.Genetic regulation of development in Sorghum bicolor (#x02168;. The ma3R Allele Disrupts Diurnal Control of Gibberellin Biosynthesis).Plant Physiology,1995,108(1):337-343.
[20]	Cowling R J,Harberd N P.Gibberellins control Arabidopsis hypocotyl growth via regulation of cellular elongation.Journal of Experimental Botany,1999,50:1351-1357.
[21]	Kraepiel Y,Rousselin P,Sotta B,Kerhoas L,Einhorn J,Caboche M, Miginiac E.Analysis of phytochrome- and ABA-deficient mutants suggests that ABA degradation is controlled by light in Nicotiana plumbaginifolia .Plant Journal,1994,6(6):665-672.
[22]	Kraepiel Y,Marree K,Sotta B,Caboche M,Miginiac E.In vitro morphogenic characteristics of phytochrome mutants in Nicotiana plumbaginifolia are modified and correlated to high indole-3-acetic acid levels.Planta,1995,197(1):142-146.
[23]	Kurapov P B,Skorobogatova I V,Sal#x02019;Nikova E I,Sorkina G L,Siusheva A G.Diurnal cycles of endogenous phytohormones in barley.Izvestiia Akademii Nauk Seriia Biologicheskaia,2000,1-2(1):108-114.
[24]	Grieb S F,Imani J,Nezamabadi M K,Arnoldt-Schmitt B,Neumann K H.Changes in soluble proteins and phytohormone concentrations of cultured carrot petiole explants during induction of somatic embryogenesis ( Daucus carota L.).Journal of Applied Botany #x00026; Food Quality,1997,71:94-103.
[25]	Edwards K D,Akman O E,Knox K,Lumsden P J,Thomson A W,Brown P E,Pokhilko A,Kozma-Bognar L,Nagy F,Rand D A,Millar A J.Quantitative analysis of regulatory flexibility under changing environmental conditions.Molecular Systems Biology,2010,6(1):424.
[26]	Harmer S L.The circadian system in higher plants.Plant Biology,2009,60:357-377.
[27]	Pruneda Paz J L,Kay S A.An expanding universe of circadian networks in higher plants.Trends in Plant Science,2010,15(5):259-265.
[28]	Umezawa T.Systems biology approaches to abscisic acid signaling.Journal of Plant Research,2011,124(4):539-548.
[29]	Qian Y Q, Li D Y,Han L,Ju G S,Liu J X,Wu J Y,Sun Z Y.Inter-ramet physiological integration detected in buffalograss ( Buchloe dactyloides (Nutt.) Engelm.) under water stress.Weed #x00026; Turfgrass Science,2009,23(2):331-343.
[30]	Tallman G.Are diurnal patterns of stomatal movement the result of alternating metabolism of endogenous guard cell ABA and accumulation of ABA delivered to the apoplast around guard cells by transpiration?Journal of Experimental Botany,2004,55:1963-1976.
[31]	Qian Y Q,Li D Y,Han L,Sun Z Y.Inter-ramet photosynthate translocation in buffalograss under differential water deficit stress.Journal of the American Society for Horticultural Science American Society for Horticultural Science,2010,135(4):310-316.
[32]	Pavlov#x000e1; L,Krekule J.Fluctuation of free IAA under inductive and non-inductive photoperiods in Chenopodium rubrum. Plant Growth Regulation,1984,2(2):91-98.
[33]	Beck E,Wagner B M.Quantification of the daily cytokinin transport from the root to the shoot of Urtica dioica L.Plant Biology,1994,107(5):342-348.
[34]	Mach#x000e1; c ˇ kov#x000e1; I,Eder J,Motyka V,Jan H,Krekule J.Photoperiodic control of cytokinin transport and metabolism in Chenopodium rubrum .Physiologia Plantarum,1996,98(3):564-570.
[35]	Hare P D,Staden J V.The molecular basis of cytokinin action.Plant Growth Regulation,1997,23(1):41-78.
[36]	Thomas T H,Hare P D,Staden J V.Phytochrome and cytokinin responses.Plant Growth Regulation,1997,23(1):105-122.
[37]	翟晓朦,臧春鑫,王敏,任梦云,关潇.CO 2 浓度升高对不同秋眠型苜蓿内源激素含量的影响.草业科学,2016,33(3):442-449.

资源附件(0)

计量

PDF下载量:
文章访问数:
HTML全文浏览量:
被引次数: 0

差异光周期下野牛草相连分株内源激素的日节律

English

Circadian rhythm variation of endogenous hormones in Buchloe dactyloides connected ramets under differential photoperiod

计量

文章相关

目录