青海地区产共轭亚油酸乳酸菌分离及其对青贮全株玉米品质的影响
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图 1 ANCLA2菌株形态学鉴定
A：ANCLA2 菌落在MRS中形态特征；B：ANCLA2扫描电镜形态特征。
Figure 1. Morphological identification of the ANCLA2 strain
A：The colony characteristics of the ANCLA2 strain on the MRS；B：The structure characteristics of the ANCLA2 strain in the SEM.
表 1 ANCLA2处理对全株玉米青贮感官品质影响
Table 1 Effect of ANCLA2 on organoleptic properties of whole corn silage
处理 Treatment 项目得分 Score of item 总分
Score等级
Grade色泽 Color 气味 Odor 水分 Water 质地 Texture pH 对照组 Control 14 17 19 7 14 71 良好 Good 植物乳杆菌 LP-1
Lactobacillus plantarum LP-117 21 19 8 18 83 优质 Excellent 植物乳杆菌 LP-2
Lactobacillus plantarum LP-218 21 19 9 20 87 优质 Excellent 共轭亚油酸菌 ANCLA2-1
Lactobacillus producing conjugated linoleie acid ANCLA2-116 20 19 8 18 81 优质 Excellent 共轭亚油酸菌 ANCLA2-2
Lactobacillus producing conjugated linoleie acid ANCLA2-218 22 19 8 20 87 优质 Excellent 
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表 2 ANCLA2处理对全株玉米青贮发酵品质影响
Table 2 Fermentation sensory quality of whole corn silage
处理 Treatment 乳酸 LA/% 乙酸 AA/% 丙酸 PA/% 丁酸 BA/% 氨态氮占总氮比
NH3-N to total N ratio/%对照组 Control 5.26 ± 0.47c 1.68 ± 0.14b 0.28 ± 0.02a 0.09 4.01 ± 0.27a 植物乳杆菌 LP-1
Lactobacillus plantarum LP-16.47 ± 0.41b 1.75 ± 0.10b 0.25 ± 0.02b 0.00 3.62 ± 0.21b 植物乳杆菌 LP-2
Lactobacillus plantarum LP-27.03 ± 0.28a 1.72 ± 0.11b 0.22 ± 0.01c 0.00 3.35 ± 0.21c 共轭亚油酸菌 ANCLA2-1
Lactobacillus producing conjugated linoleie acid ANCLA2-16.51 ± 0.32b 1.83 ± 0.14a 0.27 ± 0.02a 0.00 3.68 ± 0.22b 共轭亚油酸菌 ANCLA2-2
Lactobacillus producing conjugated linoleie acid ANCLA2-27.22 ± 0.39a 1.69 ± 0.11b 0.23 ± 0.01c 0.00 3.41 ± 0.20c 文中未特别标注的物质含量均以干物质为基础，同列不同小写字母表示不同处理间差异显著(P < 0.05)。下同。
Substance content are based on dry matter if there is not particularly marked in text. Different lowercase letters within the same column indicate significant difference among different treatments at the 0.05 level; LA, lactic acid; AA, acetic acid; PA, propionic acid; BA, butyric acid; similarly for the following tables.
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表 3 产CLA乳杆菌菌剂处理对全株玉米青贮营养成分影响
Table 3 Fermentation quality of whole corn silage
组别
Treatment干物质
DM/%粗蛋白
CP/%中性洗涤纤维
NDF/%酸性洗涤纤维
ADF/%粗脂肪
EE/%共轭亚油酸
CLA/(µg·g–1)对照组 Control 25.94 ± 1.36a 8.91 ± 0.55a 44.73 ± 1.24c 25.82 ± 0.61b 3.78 ± 0.05d 1.53 ± 0.18d 植物乳杆菌 LP-1
Lactobacillus plantarum LP-124.22 ± 1.28b 8.74 ± 0.53a 45.98 ± 1.31b 27.17 ± 1.59a 3.85 ± 0.06c 2.32 ± 0.41c 植物乳杆菌 LP-2
Lactobacillus plantarum LP-223.75 ± 1.87bc 7.86 ± 0.62b 45.32 ± 1.98b 27.89 ± 1.55a 3.89 ± 0.08c 2.56 ± 0.45c 共轭亚油酸菌 ANCLA2-1
Lactobacillus producing conjugated linoleie acid ANCLA2-125.11 ± 1.35b 8.63 ± 0.53a 47.44 ± 1.15a 26.81 ± 1.58a 4.10 ± 0.09b 9.73 ± 1.25b 共轭亚油酸菌 ANCLA2-2
Lactobacillus producing conjugated linoleie acid ANCLA2-222.97 ± 1.56c 8.12 ± 0.52b 45.96 ± 1.26b 27.56 ± 1.51a 4.23 ± 0.10a 13.62 ± 0.94a DM, dry matter; CP, crude protein; NDF, neutral detergent fiber; ADF, acid detergent fiber; EE, ether extract; CLA, conjugated linoleic acid.
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