甘菊CBL基因的克隆与表达分析

董凤丽; 刘杰; 黄河; 张蜜; 周蕴薇; 戴思兰

doi:DOI:10.11829/j.issn.1001-0629.2013-0684

甘菊CBL基因的克隆与表达分析

董凤丽^1,2,3,
刘杰⁴,
黄河⁵,
张蜜⁵,
周蕴薇⁴,
戴思兰⁵

1.
1. 东北林业大学园林学院,黑龙江哈尔滨150040
2.
2. 黑龙江生态工程职业学院,黑龙江哈尔滨 150025
3.
4.
东北林业大学园林学院,黑龙江哈尔滨150040
5.
北京林业大学园林学院,北京 100083

基金项目:

中央高校基本科研业务费专项资金(DL13CA14)

摘要: 基于已建立的甘菊ESTs文库,检索到了CBL基因4个不同的EST序列;采用RACE技术获得了它们的全长序列,并命名为ClCBL1-4。将甘菊(Chrysanthemum lavandulifolium)ClCBL与拟南芥(Arabidopsis thaliana)的CBL进行多重序列比对发现,它们都含有4个结合钙离子的EF-hand结构域,且ClCBL1在N端有一个豆蔻酰化序列(MGCXXSK/T)。采用RT-PCR技术,分析了甘菊中CBL基因的表达与不同逆境胁迫的关系,表明甘菊CBL基因的表达在冷、干旱、热、盐及ABA胁迫中可被不同程度地诱导,而在非逆境胁迫条件下,甘菊CBL基因在根、茎、叶及花等不同器官中均有表达,且表达量相对一致。这些结果说明该家族基因参与了植物非生物逆境胁迫的抵御。

关键词:

甘菊 /
CBL基因 /
基因克隆 /
表达分析

English

Cloning and expression analysis of CBL genes from Chrysanthemum lavandulifolium

1.
1. College of Landscape Architecture, Northeast Forestry University, Harbin 150040, China
2.
2. Heilongjiang Ecological Engineering Training College, Harbin 150025, China
3.
4.
College of Landscape Architecture, Northeast Forestry University, Harbin 150040, China
5.
College of Landscape Architecture, Beijing Forestry University, Beijing 100083, China

Received Date: 2013-12-10
Publish Date: 2014-07-14

Abstract: Four ESTs encoding CBL from Chrysanthemum lavandulifolium were identified in our previous study.Four CBL genes were isolated from C.lavandulifolium and named after ClCBL1-4 using RACE method. The multiple sequence alignment analysis of Arabidopsis thaliana and C. lavandulifolium CBL sequences suggested that all of the ClCBL1-4 proteins coding sequence contained four EF-hand motifs and the ClCBL1 protein coding sequence had N-terminal myristoylation sequence. The expression patterns of ClCBLs were analysis under salt,drought,cold,heat and ABA treatments as well as under normal conditions in different developmental stages. The results suggested that ClCBLs might play important roles in C. lavandulifolium’s responses to abiotic stresses because ClCBLs can be induced under different stresses.

Keywords:

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参考文献(1)

[1]

Reddy V S,Reddy A S N.Proteomics of calcium-signaling components in plants[J].Phytochemistry.2004,65(12):1745-1776Batistic O,Kudla J.Integration and channeling of calcium signaling through the CBL calcium sensor/CIPK protein kinase network[J].Planta.2004,219(6):915-924Kolukisaoglu U,Weinl S,Blazevic D,Batistic O,Kudla J.Calcium sensors and their interacting protein kinases:Genomics of the Arabidopsis and rice CBL-CIPK signaling networks[J].Plant Physiology.2004,134(1):43-58Weinl S,Kudla J.The CBL-CIPK Ca2+-decoding signaling network:function and perspectives[J].New Phytologist.2009,184(3):517-528Cheong Y H,Kim K N,Pandey G K,Gupta R,Grant J,Luan S.CBL1,a calcium sensor that differentially regulates salt,drought,and cold responses in Arabidopsis[J].Plant Cell.2003,15(8):1833-1845Pandey G K,Cheong Y H,Kim K N,Grant J J,Li L,Hung W,D’Angelo C,Weinl S,Kudla J,Luan S.The calcium sensor calcineurin B-like 9 modulates abscisic acid sensitivity and biosynthesis in Arabidopsis[J].Plant Cell.2004,16(7):1912-1924Xu J,Li H D,Chen L Q,Wang Y,Liu L L,He L,Wu W H.A protein kinase,interacting with two calcineurin B-like proteins,regulates K+ transporter AKT1 in Arabidopsis[J].Cell.2006,125(7):1347-1360黄河,王琳琳,王顺利,曹华雯,戴思兰.适于cDNA-AFLP的甘菊RNA快速高效提取方法.中国观赏园艺研究进展.北京:中国园艺学会,2009:180-188.Albrecht V,Weinl S,Blazevic D,D’Angelo C,Batistic O,Kolukisaoglu ,Bock R,Schulz B,Harter K,Kudla J.The calcium sensor CBL1 integrates plant response to abiotic stresses[J].The Plant Journal.2003,36(4):457-470张和臣.逆境条件下胡杨CBL-CIPK信号途径转导的分子机制研究.北京:北京林业大学,2010.Chen J H,Sun Y,Sun F,Xia X L,Yin W L.Tobacco plants ectopically expressing the Ammopiptanthus mongolicus AmCBL1 gene display enhanced tolerance to multiple abiotic stresses[J].Plant Growth Regulation.2011,63(3):259-269

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甘菊CBL基因的克隆与表达分析

English

Cloning and expression analysis of CBL genes from Chrysanthemum lavandulifolium

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