Abstract: The 160 plants of Seriphidium transiliense with the different geographical population growing at different terrains was used to screen the genetic diversity by SRAP technical analysis for providing the useful information for utilization this plant resource. The results of this study showed the percentage of polymorphism loci of S. transiliense population was 78.43. This study also indicated that the obvious demarcation point was not found among the different geographical population of S. transiliense, implying that the difference in geographical distance between S. transiliense populations did not affect the hereditary substance of S. transiliense. However, 160 plants were clustered into two groups, and one group consisted of sunny slope and plain populations, the other group consisted of shady slope and hilly populations, indicating that terrain showed a greatly effect on the hereditary substance of S. transiliense.