Abstract: Tibetan Plateau is known as the Roof of the World with harsh，unique climatic characteristics and geographic conditions and cold, high solar radiation and strong ultraviolet light et al，and has profound influences on the morphology, photosynthetic pigments content and photosynthetic process of leaf of alpine plants. The plateau climate is important to photosynthetic pigments content, the constituent and photosynthesis of leaf, few studies have been focused on the relationship between photosynthetic pigments and photosynthesis of alpine plants in the third pole. In this research，14 plants of 9 species were selected and photosynthetic pigments content and the chlorophyll fluorescence of characteristics of leaf in the alpine meadow ecosystem were measured. The results showed that 1) To some extent，the chlorophyll content of alpine plants can reflect the photosynthetic capacity of leave; 2) The different composition proportion of photosynthetic pigments is closely related to photosynthetic physiology of plants.