Abstract: The formula of ecoorganic soilless matrix for cultivation is the focus of formula of soilless matrix for cultivation in the future. Based on previous studies, the new four formulas were constructed by combining the peat, plant ash, litter, humus and appropriate amount of small particles of poly to a certain volume ratio to substitute for soil cultivation in this study. This study not only screened out the most suitable formula of ecoorganic soilless matrix for E. dahuricus growth at its seedling stage by adding basic manure into four ecoorganic soilless matrixes, but also provides a basis theory for the formulation. The results of this study showed that the higher nutrient content benefited seedling growth of E. dahuricus when the ecoorganic soilless matrix met the requirements of an ideal matrix. The emergence of E. dahuricus was closely related to the physical property of the matrix, but was not related to nutrient composition of the matrix. This study showed that A3 matrix was best ecoorganic soilless matrix for cultivation, in which the ratio of humus, litter and plant ash was 6∶2∶2.