Research progresses in Medicago truncatula genetic transformation

Leguminous crops have widespread economic importance as protein sources for humans and animals. As the legume model plant, the establishment and improvement of the genetic transformation system of Medicago truncatula are of great significance to the promotion of legume genetic engineering research and functional genomics studies. We summarized here the research progress on genetic transformation of M.truncatula in R108, Jemalong 2HA, Jemalong J5, Jemalong A17 and Jemalong M9-10a, in terms of transformation methods, explants, bacterial strains, vectors and culture mediums. Moreover, the existing problems and development tendency were summarized, expecting to provide theoretical basis for the further improvements of legume genetic transformation system.