Tissue culture and rapid propagation of Polygonum viscosum stems stems with axillary buds

In order to establish the tissue culture and rapid propagation system of Polygonum viscosum, the present study explored the impacts of different concentrations of hormone on axillary buds induction, axillary buds proliferation and root induction with the stems with axillary buds of P.m viscosum as explant and MS as the basic medium. The results showed that the optimal medium for axillary bud induction was MS medium with 2.0 mg·L-1 6-BA purine and 0.1 mg·L-1 NAA which had the highest induction rate (88.89%). The optimal medium for axillary buds proliferation was MS medium with 2.0 mg·L-1 6-BA, 0.3 mg·L-1 NAA acid and 0.5 mg·L-1 KT which had the highest coefficient of multiplication (5.17). The optimal medium for root induction was 1/2 MS medium with 0.7 mg·L-1 NAA which had the highest root induction rate (98.89%).Plants grew very well after transplanting with the survival rate of 93.33%.This research supplied favorable information for P.viscosum rapid propagation and genetic engineering.