Plant regeneration system of wild Kentucky bluegrass in Longxi, Gansu Province

The present research studied the influence of different hormones and their concentrations on callus induction and differentiation of mature seeds from wild Kentucky bluegrass in Longxi, Gansu. Callus induction rate had significant difference (P0.05) with different regeneration medium and with different concentrations of 2,4-D. MS medium supplemented with 1.0 mg·L-1 2,4-D was optimum for callus induction which had a callus induction rate of 41.40%. When 2, 4-D used alone, callus induction had higher frequency and better quality compared with the combination of 2,4-D and 6-BA. MS medium supplemented with 2 mg·L-1 6-BA was optimum for callus differentiation. The green plant frequency increased significantly (P0.05) after adding 0.5 mg·L-1 NAA and 0.2 mg·L-1 2,4-D to MS medium with 2 mg·L-1 6-BA which was 22.88% and 19.72%, respectively. The optimum medium for rooting differentiation was the same as the medium for callus differentiation which has 100% rooting frequency.