Research on pollen viability and stigma receptivity of Adonis amurensis

In order to study pollen morphology, pollen viability and stigma receptivity, an investigation on pollen and stigma of Adonis amurensis in Heilongjiang, China was conducted. The relationships between pollen viability, stigma receptivity and pollination success were also analyzed. The results showed that the best medium for pollen germination was 10% sucrose + 0.1 g·L-1 H3BO3+0.1 g·L-1 Ca (NO3)2. Pollen viability maintained more than 70% at the first three days of blossom. The highest pollen viability was observed at the second day and decreased significantly since the fourth day. Pollen viability was 31.92% at the end of florescence. Stigma had weak receptivity ability at the first day and strong receptivity ability since the second day which maintained 2~3 days. Pollen had germination ability at the first day of blossom and anther crack delayed 2~3 days which provided the opportunity for cross-pollination. At the same time, there was the possiblity of self-fertilize for the environmental constraints and space advantage of self pollen.