Cluster buds induction and plant regeneration technology of Iris ensata embryo

The embryos of Iris ensata were used as explants to study cluster buds induction and plant regeneration. The optimal culture medium for cluster buds induction was MS medium supplemented with 1.0 mg·L-1 6-BA purine, 0.6 mg·L-1NAA, and 2.0 mg·L-1KT. The greatest multiplication coefficient for bud induction (9.61) was achieved after induction for 60 days. The optimal root induction culture medium was MS medium supplemented with 0.5 mg·L-1 NAA which had a rooting rate of 100%. The present study facilitated effective development and utilization of I. ensata germplasm, and provided sterile technology for germplasm conservation, breeding and other asexual reproduction researches.