Cloning of calmodulin gene from Embellisia astragali

The yellow stunt and root rot of standing milkvetch disease caused by Embellisia astragali is one of the major factors in the pasture degradation. Calmodulin is significant protein in Ca2+ dependent signaling pathway. The present study was aimed to clone calmodulin gene from E. astragali genomic DNA. The homologous DNA fragment of 539 bp was amplified from genomic DNA with primers CAL228F and CAL737R. Then, the 5 flanking sequence of 344 bp and 3 flanking sequence of 729 bp were amplified using HiTAIL PCR. Finally, the 1 290 bp fulllength DNA sequence was assembled and 840 bp fulllength cDNA sequence was amplified with primers CaMF and CaMR. There were 4 introns in DNA fulllength sequence which had the typical characteristics of ATAG. The polypeptide with 149 amino acid was translated from cDNA sequence.