Cloning and expression analysis of Actin gene fragment from halophyte Halogeton glomeratus

In the present study, the Actin gene fragment was amplified by reverse transcription polymerase chain reaction (RT-PCR) from total RNA extracted from leaves of H. glomeratus with designed degenerated primers based on the conserved sequences of Actin genes from chenopodiaceous plants. The Actin gene fragments were cloned into pMD19-T vector, sequenced using positive clone and registered in GenBank (accession number：KF699314). The sequence results indicated that the length of Actin gene fragment was 598 bp which encoded 198 amino acids and homology of amino acid sequences translated from nucleotides acids from H. glomeratus and other plants was 94% which suggested the high conservation of this gene. There was no difference between Actin gene expressions in different organs of H.glomeratus based on quantitative RT-PCR (qRT-PCR) analysis which suggested that Actin gene can be selected as internal control gene for study the expression of other salttolerant genes of H. glomeratus.