Identification of lactic acid bacteria and fermentation characteristics ofmixed ensilages of corn stover and cabbage waste

To explore the feasibility of corn stover (CS) and cabbage waste (CW) mixed silage, the effects of different ratio on ensilages quality were studied to assess the best ration of two materials. Meanwhile, conventional identification methods and 16SrRNA sequence analysis were employed to analyze the lactic acid bacteria diversity from the optimum mass ratio of CS and CW ensilages. The results showed that the pH value of all the mixed silages were lower than CS solely ensilages (SECS, P0.05) which mean that the quality of mixed ensilages were better than CS sole ensilages. The ME5 group was the optimal mass ratio in mixensiling groups. A total number of 12 lactic acid bacteria were isolated from the ME5 group, which belonged to the three different generas (Lactobacillus, Pediococcus and Enterococcus). Among these isolates, Lactobacillus and Pediococcus were the predominant strains (91.7% of the total isolates). They were composed of 1 strain of L. brevis, 3 strains of L. plantarum, 7 strains of P. pentosaceus and 1 strain of E. hirae which mainly were homofementative of lactobacillus. The research result supported that the way of CS and CW mixed ensilages was feasible and had laid the foundation for preparing an excellent lactic acid bacteria addictive for silage.