Influence of Thidiazuron on dormancy release of Leymus chinensis seed and plant regeneration

In the experiment, dormancy release of Leymus chinensis seed and plant regeneration is studied using plant growth regulator TDZ with different concentrations, and ultramicro observations are conducted on callus. The results showed that 0.02 mg·L-1 TDZ cold soaking has the best effect on dormancy release of L. chinensis seed and embryonic callus induction. The germination rate and the healing rate are 11% and 15.5%, respectively, the best media component formula for L. chinensis callus differentiation is MS+TDZ 0.5 mg·L-1+CH 2.0 g·L-1, the differentiation rate and green shoot differentiation rate are 56.7% and 423.3%, respectively. Otherwise, transmission electron microscope (TEM) observations found that the embryonic callus induced by TDZ cold soaking, L. chinensis seeds contain more rich contents and are more conductive to body embryo formation than that of non-embryonic callus cell plasmid. After acclimatization and transplanting, the survival rate of the multiple shoots through 3 times of subculture with 1/2 MS rooting media is 100%.