Optimization of seed hydropriming conditions for Medicago sativa

Failure of seedling establishment is one of the main reason limiting cultivation of Medicago sativa in arid rain-fed region of Loess Plateau. Seed priming has been proven to be an effective method for enhancing stress tolerance of seeds at the germination stage; and consequently increased seedling establishment. This study determined the optimal conditions for hydropriming of M. sativa cv. “Longdong” by manipulating priming temperature (10, 15, 20 ℃), duration (12, 24, 36, 48 h) and water addition (90%, 120%, 150% seed weight). The results showed that most priming treatments could significantly increased germination rate and seedling growth, but showed no effect on final germination percentage. The optimal response to hydropriming was achieved at 10 ℃ for 36 h with 90% water of original seed weight.