Tissue culture and rapid propagation of Salvia hispanica

In order to establish the tissue culture and rapid propagation system of Salvia hispanica, the present study explored the impacts of different concentrations of hormone on callus induction, proliferation and root induction with the mature seed of S. hispanica as explant and 1/2MS as the basic medium. The results showed that the optimal medium for callus induction was 1/2MS medium with 10.0 mg·L-1 6-BA, 1.0 mg·L-1 NAA and 0.4 mg·L-1 ZT, which had the highest induction rate of 66.5%. The optimal medium for the proliferation was 1/2MS medium with 3.0 mg·L-1 6-BA and 0.5 mg·L-1 NAA, with the highest multiplication coefficient of 7.69. The optimal medium for the root induction was 1/2MS medium with 0.5 mg·L-1NAA, which had the highest root induction rate of 100% and the average rooting coefficient of 5.68. Plants grew very well after transplanting with a survival rate of 97%.