Construction of DNA fingerprinting of Stylosanthes standard cultivars for DUS testing

DNA fingerprinting of Stylosanthes standard cultivars for distinctness, uniformity and stability (DUS) testing was constructed to facilitate the identification of Styosanthes cultivars. The genetic diversity analysis and DNA fingerprinting construction of 15 Stylosanthes standard cultivars for DUS testing were performed using 25 SSR markers. The results indicated that there were total of 132 alleles were generated from 25 SSR markers among these 15 standard cultivars. The number of alleles generated from each SSR marker ranged from 3~9, with an average of 5.280 alleles. The polymorphic information content per SSR marker varied from 0.393 to 0.847, with an average of 0.638. The 15 standard cultivars could be successfully distinguished by DNA fingerprinting, which was constructed based on these 25 SSR markers. The DNA fingerprinting QR code for each Stylosanthes standard cultivars was constructed. The results could provide important reference for application of SSR marker in Stylosanthes DUS testing.