Study on genetic characteristics of spotted gene in spotted colored pea

In order to clarify genetic characteristics of seed coat color in spotted colored pea, three pea cultivars were used as experimental materials, i.e. Mawan1 (spotted seed coat, purple flower and semi-leafless), Zhongwan 6 (no dots on seed coat, white flower and common leaf) and 90-PE-10 (no dots on seed coat, white flower and semi-leafless). Reciprocal cross combinations, i.e. Mawan 1 and Zhongwan 6, Mawan1 and 90-PE-10 were set. Progenies of parents, F1 and F2 were observed under field condition and statistical analyses were undertaken to the obtained data. The results showed that F1 displayed spotted seed coat in the two reciprocal cross combinations, and the F2 progenies segregated in a 3∶1 ratio indicating monogenic inheritance. Spotted seed coat is dominant over the no dots on seed coat. In two pairs of relative characters experiments, two kinds of recombinants (spotted seed coat and white flower, no dots on seed coat and purple flower) were not found in F2 progenies in the two reciprocal cross combinations. The gene of spotted seed coat is tightly linked to the gene of purple flower. It is convenient to select for seed color or flower color using the genetic marker. The gene of spotted seed coat located on chromosome 1 in pea, is different from F and Fs, spotted genes, located on chromosome 3 and chromosome 5 respectively.