Effects of chemical mutagens on chromosome doubling of Agropyron mongolicum

Lu-lu Zhou; Bing-zhe Fu; Dong-mei Xu; Xue-qin Gao

doi:10.11829/j.issn.1001-0629.2015-0519

Pratacultural Science > 2016 > 10(5): 897-906. > DOI: 10.11829/j.issn.1001-0629.2015-0519

Lu-lu Zhou, Bing-zhe Fu, Dong-mei Xu, Xue-qin Gao. Effects of chemical mutagens on chromosome doubling of Agropyron mongolicum[J]. Pratacultural Science, 2016, 10(5): 897-906. DOI: 10.11829/j.issn.1001-0629.2015-0519

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Effects of chemical mutagens on chromosome doubling of Agropyron mongolicum

Lu-lu Zhou^1,2,3,
Bing-zhe Fu^1,2,3,
Dong-mei Xu^1,2,3,
Xue-qin Gao⁴

1.
1. Grassland Science Institute, Agricultural College, Ningxia University, Yinchuan 750021, China
2.
2. Breeding Base for State Key Labotatory of Land Degradation and Ecological Restoration in Northwest China, Ningxia University, Yinchuan 750021, China
3.
4.
Science and Technology Department, Ningxia University, Yinchuan 750021, China

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Received Date: September 15, 2015
Published Date: May 19, 2016

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Abstract

Abstract

In order to improve the forage quality of Agropyron mongolicum and screen the best chromosome doubling treatment, the seeds of A. mongolicum were treated with different concentrations of Colchicine (0.02%,0.05%,0.08%,0.11%,0.14%+1.5% DMSO) and Trifluralin (1,5,10,15,20 μmol·L-1) and the morphology and chromosome number were identified after treatment. The results showed that the surviving plants after treated were mixoploid of diploid and tetraploid. The treatment with 0.11% colchicine 1.5% DMSO for 20 h had the highest mutation rate of 50% and the survival rate of 40%. The treatment with 1 μmol·L-1 trifluralin for 6 h had significant mutation effect with probability of 38.46%. These results provide theoretical and experimental basis for further screening the chromosome doubling plants of A. mongolicum.
- Agropyron mongolicum,
- mutagen,
- chromosome doubling,
- ploidy identification

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References

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