The analysis of differential proteins in lily bulbs during dormancy breaking

Lily bulb dormancy was broken during low temperature storage, Fluorescent difference gel electrophoresis, MALDI-TOF/TOF and bioinformatic technology were used in the experiment. A total of 31 differentially expressed proteins were separated by using fluorescent difference gel electrophoresis. Among these proteins, there were 15 proteins up-regulated and 16 proteins down-regulated in the dormancy breaking bulbs. These proteins were classified into 6 categories by function. Comprehensive analysis showed that these proteins were mainly stress protein,they may involved in metabolic process of the bulbs, and regulate the bulb dormancy breaking. According to the expression differences of the proteins in dormant and dormancy breaking bulbs, several proteins identified might be related to the process of bulb dormanc.Stress proteins had high expression in dormant bulbs, and proteolytic enzymes had high expression in dormancy breaking bulbs. This research had set the stage for further understanding of the bulb dormancy mechanisms in lily.