Genetic diversity of endophytic bacteria in nodule of Sphaerophysa salsula

Sphaerophysa salsula is an important legume distributed in northwestern China. In order to better understand the diversity and phylogeny of endophytic bacteria collected from nodules of Swainsonia salsula in different regions of northwestern China, genetic diversity of these isolated endophytic bacteria was estimated using 16S rDNA PCR-RFLP and 16S rDNA sequencing. The results showed that these 115 bacteria strains had 28 genetic types which were further identified by 16S rDNA sequencing. These strains belonged to the following genera: Mesorhizobium, Rhizobium, Sinorhizobium, Paracoccus, Sphingomonas, Inquilinus, Pseudomonas, Serratia, Mycobacterium, Nocardia, Streptomyces, Paenibacillus, Brevibacillus, Lysinibacillus, Staphylococcus, Bacillus. These results demonstrated that endophytic bacteria in nodule had rich genetic diversity in S. salsula.