Optimization of anthocyanidin extraction from Anemone obtusiloba using response surface methodology

In this study, we used the sepals of Anemone obtusiloba as experimental material for extraction of anthocyanidin using response surface methodology. Four factors were selected in this experiment: the material liquid mass concentration, pH, extraction time, and extraction temperature. We selected the light absorption value of the anthocyanidin of A. obtusiloba as a response surface to optimize the extraction conditions of anthocyanidin. Our results confirmed that the most efficient extraction of anthocyanidin was achieved under the following conditions: a material liquid mass concentration of 70 g·mL-1, an extraction temperature of 61 ℃, an extraction time of 87 min, and pH 3.8. Under these conditions, we conclude that the best light absorption value of A. obtusiloba is 0.154 5.