Study on callus inductions of Lvzhou No.3 ——a new material of Arundo

In order to induce callus, the experiment was conducted to study the sterilization effects of NaClO (or Antiformin) and HgCl2 on stems respectively by using Lvzhou No.3 as materials. We took the sterile stems, germs and young leaves derived from the sterile stems as explants, different concentrations of hormones and 1.0 g·L-1 PVP were used for callus induction. The results showed that the best sterilization effect is to use 0.1% HgCl2 to sterilize for 18 min and the pollution rate was 13%. While NaClO cannot be sterilized effectively, and it also has toxic effect on stem segment. The culture mediums with best induction effect were MS+0.5 g·L-1 6-BA, 1.0 g·L-1 PVP and 3.0 g·L-1 2,4-D. In three kinds of explants, the order of induction rate was germs>stems>young leaves. 1.0 g·L-1 PVP can be used to promote the induction rate of buds significantly, making 100% callus formation.