Lactic acid bacteria diversity analysis and screening of superior thermotolerant strains from corn stover and cabbage waste mixed-silages

Morphological observation, physiological and biochemical tests, and the 16S rRNA sequence analysis methods were used to analyse lactic acid bacteria (LAB) diversity in mixed silage of corn stover and cabbage, and the thermotolerant strains were screened through with temperature and pH as the restrictive indicators. The results showed that 12 LAB strains were isolated from mixed silage, which were classified into Lactobacillus and Pediococcus, respectively. Specifically, one strain (LB-1) was Lactobacillus plantarum, six strains (LB-2, LB-4, LB-7, LB-8, LB-9 and LB-11) were Pediococcus pentosaceus, three strains (LB-5, LB-6 and LB-12) were Lactobacillus brevis, and two strains (LB-3 and LB-10) were Lactobacillus paracasei. The characteristics of LB-3 and LB-8 included high-temperature resistance, acid-resistance and higher acid-production ability. Therefore, these two lactic acid bacteria can be used as inoculants for silage forage.