Establishment and optimization of a suspension culture system for culturing Asarum heterotropoides

Using the callus induced by young petioles of Asarum heterotropoides, a suspension culture system was established. Then the effects of the amount of inoculum, lighting time, amount of culture fluid, speed of rotation, and concentrations of sucrose and plant growth regulators on the cell suspension culture of A. heterotropoides were studied. The results showed that the optimal conditions for callus suspension culture were 2 g calli inoculated in 100 mL 1/2 MS liquid medium containing 0.6 mg·L-1 6-BA and 0.3 mg·L-1 NAA, a rotation speed of 150 r·min-1, 30 g·L-1 sucrose, and exposure to whole-day light. The growth curve of suspension cells of A. heterotropoides was S-type. The best harvest time of suspension cells was 15 d. Through the suspension culture, the cell masses and various forms of single cells could be obtained rapidly. These findings provide the basis for large-scale culture of A. heterotropoides suspension cells so as to enhance the application value of A. heterotropoides.