Analysis of EMS and 60Co-&#x003b3; mutagenesis of Dianthus spiculifolius seeds

Shu-fang Gong; En-hui Liu; Tong-tong Jiang; Ai-min Zhou; Jin-gang Wang

doi:10.11829/j.issn.1001-0629.2017-0053

Pratacultural Science > 2017 > 11(11): 2226-2234. > DOI: 10.11829/j.issn.1001-0629.2017-0053

Shu-fang Gong, En-hui Liu, Tong-tong Jiang, Ai-min Zhou, Jin-gang Wang. Analysis of EMS and 60Co-γ mutagenesis of Dianthus spiculifolius seeds[J]. Pratacultural Science, 2017, 11(11): 2226-2234. DOI: 10.11829/j.issn.1001-0629.2017-0053

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Analysis of EMS and 60Co-γ mutagenesis of Dianthus spiculifolius seeds

College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, Heilongjiang, China

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Received Date: January 29, 2017
Published Date: November 09, 2017

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Abstract

Abstract

In order to develop new varieties of Dianthus spiculifolius, we mutagenized seeds using EMS and 60Co-γ rays. Treatment of seeds for 16 h with 1% EMS, close to the half-lethal dose, resulted in a mutation rate of 7.0%. Seeds treated with 300 Gy 60Co-γ rays (dose rate 0.1 Gy·min-1, close to the half-lethal dose, resulted in a mutation rate of 4.2%. We obtained 1 038 phenotypic mutants in the M1 generation, of which 95 were derived from seeds treated with EMS, and 943 were obtained following irradiation of the seeds. This study has enriched the germplasm resources of D. spiculifolius and has provided a technical reference for the mutagenesis of other Dianthus species.
- seed,
- mutagenesis,
- mutation rate,
- ethyl methane sulfonate,
- 60Co-γ,
- rays,
- mutant,
- Dianthus

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References

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