Comparison of methods for detecting aflatoxins B1 and M1 in milk

Yu-fen Luo; Xiu-xiu Weng; De-fu Tang; Jian-zhong Pan; Fang Nian

doi:10.11829/j.issn.1001-0629.2017-0104

Pratacultural Science > 2017 > 11(12): 2546-2553. > DOI: 10.11829/j.issn.1001-0629.2017-0104

Yu-fen Luo, Xiu-xiu Weng, De-fu Tang, Jian-zhong Pan, Fang Nian. Comparison of methods for detecting aflatoxins B1 and M1 in milk[J]. Pratacultural Science, 2017, 11(12): 2546-2553. DOI: 10.11829/j.issn.1001-0629.2017-0104

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Comparison of methods for detecting aflatoxins B1 and M1 in milk

1.
Faculty of Science, Gansu Agricultural University, Lanzhou 730070, Gansu, China
2.
College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730000, Gansu, China
3.
Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, Gansu, China
4.
Lanzhou Food and Drug Inspection, Lanzhou 730000, Gansu, China

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Received Date: March 07, 2017
Revised Date: April 20, 2017
Published Date: December 11, 2017

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Abstract

Abstract

Immunoaffinity column-high performance liquid chromatography (HPLC) and enzyme-linked immunosorbent assay (ELISA) methods were adopted to determine aflatoxins (AF) B1 and M1 in milk. The recovery, detection limit, and precision of these two methods were compared in this study. The results showed that, for HPLC, the detection limits of AFB1 and AFM1 were 0.02 and 0.01 μg·L-1, respectively, while for ELISA, the detection limits of AFB1 and AFM1 were 0.05 and 0.02 μg·L-1, respectively. Standard solutions of AFB1 (0.003, 0.006, 0.012, and 0.024 μg·kg-1) and AFM1 (0.005, 0.01, 0.025, and 0.05 μg·kg-1) were added into blank milk samples and the results of recovery experiments indicated that, for HPLC, the recoveries of AFB1 and AFM1 were 77.58%~82.81% and 77.51%~82.23% with relative standard deviations of 1.93% and 3.51%, respectively. For ELISA, the recoveries of AFB1 and AFM1 were 77.28%~79.11% and 75.40%~76.34% with relative standard deviations of 2.20% and 3.80%, respectively. The recovery of AFB1 did not significantly differ between the two methods (P>0.05). However, the recovery of AFM1, when the amount added was more than 0.025 μg·kg-1, was significantly higher by HPLC than by ELISA (P1, the accuracy of HPLC was better than that of ELISA.
- high-performance liquid chromatography method,
- enzyme-linked immunosorbent assay,
- comparison,
- recovery,
- precision

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References

[1]	张艺兵,张鹏,宋小岩.食品供应中真菌毒素的风险分析方法.食品工业科技,2002,23(7):76-83.
[2]	Zhang Y B,Zhang P,Song X Y.Risk analysis method of mycotoxins in food supply.Science and Technology of Food Industry,2002,23(7):76-83.(in Chinese)
[3]	尚沁沁,熊平文,梁权,饶正华,王雄,果旗,余东游.免疫亲和柱净化-高效液相色谱法测定饲料中的黄曲霉毒素.饲料研究,2015(22):55-60.
[4]	Shang Q Q,Xiong P W,Liang Q,Rao Z H,Wang X,Guo Q,Yu D Y.Determination of aflatoxins in feed by immunoaffinity column purification-high performance liquid chromatography method.Feed Research,2015(22):55-60.(in Chinese)
[5]	王蕾.牛奶中黄曲霉毒素M1检测方法的建立及饲料中黄曲霉毒素对牛奶品质的影响.郑州:河南农业大学硕士学位论文,2008.
[6]	Wang L.Studies on detecting aflatoxin M1 in milk and effects of aflatoxin in feed on milk quality.Master Thesis.Zhengzhou:Henan Agricultural University,2008.(in Chinese)
[7]	李秀缺,张薇,张爱菊,张秋生.花生中黄曲霉毒素的防控及去除方法.食品工程,2010(2):25-27,50.
[8]	Li X Q,Zhang W,Zhang A J,Zhang Q S.Prevention and control of aflatoxin in peanuts and removal methods.Food Engineering,2010(2):25-27,50.(in Chinese)
[9]	孟昭赫,张国柱,宋圃菊.真菌毒素研究进展.北京:人民卫生出版社,1979:14-18.
[10]	Meng Z H,Zhang G Z,Song P J.Fungaltoxin Research Progress.Beijing:People’s Medical Publishing House,1979:14-18.(in Chinese)
[11]	Alborzi S,Pourabbas B,Rashidi M,Astaneh B.Aflatoxin M1 contamination in pasteurized milk in Shiraz (south of Iran).Food Control,2006,17(7):582-584.
[12]	田维荣,代兴红,胡萍.牛奶中黄曲霉毒素的危害及质量控制.畜牧与饲料科学,2013,34(2):36-37.
[13]	Tian W R,Dai X H,Hu P.Harms and quality control of aflatoxin in milk.Animal Husbandry and Feed Science,2013,34(2):36-37.(in Chinese)
[14]	Decastelli L,Lai J,Gramaglia M,Monaco A,Nachtmann C,Oldano F,Ru Y M,Sezian A,Bandirola C.Aflatoxins occurrence in milk and feed in Northern Italy during 2004-2005.Food Control,2007,18(10):1263-1266.
[15]	陈瑞春,段文仲,吕红英,郭春海,王丹娜,刘凌云.免疫亲和层析净化-高效液相色谱法同时测定牛奶和奶粉中黄曲霉毒素B1、B2、G1、G2、M1、M2.食品科技,2011,36(8):300-304.
[16]	Chen R C,Duan W Z,Lyu H Y,Guo C H,Wang D N,Liu L Y.Determination of aflatoxin B1,B2,G1,G2,M1 and M2 in milk and milk powder by immunoaffinity chromatography and high-performance liquid chromatography method.Food Science and Technology,2011,36(8):300-304.(in Chinese)
[17]	Abbès S,Salah-Abbès J B,Bouraoui Y,Oueslati S,Oueslati R.Natural occurrence of aflatoxins (B1 and M1) in feed,plasma and raw milk of lactating dairy cows in Beja,Tunisia,using ELISA.Food Additives and Contaminants,2012,5(1):11-15.
[18]	International Agency for Research on Cancer.Some mycotoxins,naphtalene and styrene.Monographs on the evaluation of carcinogenic risk to humans.Lyon:World Health Organization,2002,82:171-300.
[19]	Diaz G J,Espitia E.Occurrence of aflatoxin M1 in retail milk samples from Bogota,Colombia.Food Additives and Contaminants,2006,23(8):811-815.
[20]	Goryacheva I Y,Karagusheva M A,Peteghem C V,Sibanda L,Saeger S D.Immunoaffinity pre-concentration combined with oncolumn visual detection as a tool for rapid aflatoxin M1 screening in milk.Food Control,2009,20(9):802-806.
[21]	付春丽,刘文静,高腾云.霉菌毒素对奶牛的影响及其控制措施.草业科学,2015,32(9):1500-1507.
[22]	Fu C L,Liu W J,Gao T Y.Effects of mycotoxins on dairy cows and control measures.Pratacultural Science,2015,32(9):1500-1507.(in Chinese)
[23]	European Commission Regulation(EC) No 1881/2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs.Official Journal of European Union,2006,364:5-24.
[24]	US Food and Drug Administration (1996) Sec.527.400 whole milk,low fat milk,skim milk-aflatoxin M1 (CPG 7106.210).FDA compliance policy guides,Washington,DC,1996:219.
[25]	Vargas E A,Preis R A,Castro L,Silva C M.Co-occurrence of aflatoxins B1,B2,G1,G2,zearalenone and fumonisin B1 in Brazilian corn.Food Additives and Contaminants,2001,18(11):981-986.
[26]	Sarmehmetoglu B,Kuplulu O,Haluk Celik,T.Detection of aflatoxin M1 in cheese samples by ELISA.Food Control,2004,15(1):45-49.
[27]	Khayoon W S,Saad B,Lee T P,Salleh B.High performance liquid chromatographic determination of aflatoxins in chilli,peanut and rice using silica based monolithic column.Food Chemistry,2012,133(2):489-496.
[28]	马志科,昝林森.黄曲霉毒素危害、检测方法及生物降解研究进展.动物医学进展,2009,30(9):91-94.
[29]	Ma Z K,Zan L S.The research progress of harm,detection methods and biodegradation about aflatoxins.Progress in Veterinary Medicine,2009,30(9):91-94.(in Chinese)
[30]	周贻兵,刘利亚,李磊,林野,李雪春.免疫亲和柱净化-光化学衍生高效液相色谱荧光法测定植物油中黄曲霉毒素B1的含量.中国油脂,2014,39(6):92-94.
[31]	Zhou Y B,Liu L Y,Li L,Lin Y,Li X C.Determination of aflatoxin B1 in vegetable oil by HPLC-FLD with photochemical derivation after immunoaffinity column purification.China Oils and Fats,2014,39(6):92-94.(in Chinese)
[32]	张晓彤.液相色谱检测方法.北京:化学工业出版社,2000:12-13.
[33]	Zhang X T.Liquid Chromatography Method.Beijing:Chemical Industry Press,2000:12-13.(in Chinese)
[34]	Lee D H,Lee K-G.Analysis of aflatoxin M1 and M2 in commercial dairy products using high-performance liquid chromatography with a fluorescence detector.Food Control,2015,50:467-471.
[35]	胡宏灿,韩飞,林悦楷,卢任杰.HPLC与ELISA法测定牛奶中黄曲霉毒素M1的比较研究.化学分析计量,2013,22(1):48-50.
[36]	Hu H C,Han F,Lin Y K,Lu R J.Comparison of HPLC and ELISA method in detection of aflatoxin M1 in milk.Chemical Analysis and Meterage,2013,22(1):48-50.(in Chinese)
[37]	周贻兵,林野,李磊,王娅芳,刘利亚.高效液相色谱法测定牛奶中黄曲霉毒素M1的含量.食品研究与开发,2014,35(15):96-98.
[38]	Zhou Y B,Lin Y,Li L,Wang Y F,Liu L Y.Determination of aflatoxin M1 content in milk by high-performance liquid chromatography.Food Research and Development,2014,35(15):96-98.(in Chinese)
[39]	马海华,孙楫舟,甄彤,张元,夏善红.我国国家标准和行业标准中黄曲霉毒素测定方法综述.食品工业科技,2016,37(6):360-366.
[40]	Ma H H,Sun J Z,Zhen T,Zhang Y,Xia S H.A review on aflatoxins determination methods in China’s national and industry standards.Science and Technology of Food Industry,2016,37(6):360-366.(in Chinese)
[41]	黄亚伟,魏光,王若兰,罗莉.乳品中黄曲霉毒素M1检测方法研究进展.食品安全质量检测学报,2014,5(3):770-775.
[42]	Huang Y W,Wei G,Wang R L,Luo L.Review on detection methods of aflatoxin M1 in dairy products.Journal of Food Safety and Quality,2014,5(3):770-775.(in Chinese)
[43]	卢安根,莫建光,杜寒春,陈秋虹.酶联免疫法快速检测牛奶及其制品中的黄曲霉毒素B1.广西科学院学报,2010,26(3):306-308.
[44]	Lu A G,Mo J G,Du H C,Chen Q H.Application of ELISA for rapid detection aflatoxin B1 in milk and dairy products.Journal of Guangxi Academy of Sciences,2010,26(3):306-308.(in Chinese)
[45]	李平,谢体波,易重任,魏丽丽,王大敏,张建宇,欧翔,陈芳,罗先坤.黄曲霉毒素M1 ELISA试剂盒的检测效果研究.食品安全质量检测学报,2015,6(6):2297-2302.
[46]	Li P,Xie T B,Yi Z R,Wei L L,Wang D M,Zhang J Y,Ou X,Chen F,Luo X K.Research on detection results of aflatoxin M1 enzyme-linked immunosorbent assay detection kit.Journal of Food Safety and Quality,2015,6(6):2297-2302.(in Chinese)
[47]	姚佳,王昕,张建新,李贤良,郗存显,王国民,王雄,里南,果旗.免疫亲和柱-高效液相色谱法测定牛奶中氯霉素和玉米赤霉醇及其类似物.食品科学,2014,35(18):124-127.
[48]	Yao J,Wang X,Zhang J X,Li X L,Xi C X,Wang G M,Wang X,Li N,Guo Q.Simultaneous determination of residues of chloramphenicol,zearalanol and its analogues in milk by high performance liquid chromatography with immunoaffinity column.Food Science,2014,35(18):124-127.(in Chinese)

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