In vitro tissue culture and plant regeneration of Clematis florida ‘Blekitny Aniol’
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Abstract
In this study, aseptic Clematis florida‘Blekitny Aniol’ seedlings were obtained from stem segments with axillary buds, and callus induction and differentiation of leaves and stem segments were analyzed. The regeneration system of ‘Blekitny Aniol’ was established. The results showed that the optimal medium for the induction of axillary buds from the stem segments with axillary buds was MS+1 mg·L-16-BA+0.05 mg·L-1NAA, and the germination rate of axillary buds was 100.0%. The optimal medium for callus induction from stem segments was MS+2 mg·L-16-BA+0.01 mg·L-1NAA with a callus induction rate of 78.3%. The optimal medium for callus induction from leaf blades was MS+1 mg·L-16-BA+0.1 mg·L-1NAA with the highest callus induction rate of 81.7%. The optimal medium for callus differentiation was 1/2MS+1 mg·L-16-BA+0.05 mg·L-1NAA, and the callus differentiation rate was 25.0%. The optimal medium for adventitious budproliferation was 1/2MS+3 mg·L-16-BA+0.1 mg·L-1NAA, and the highest proliferation times was 4.94. Rooting rate of regenerated plants reached up to 70.2% on half-strength MS medium supplemented with 0.05 mg·L-1NAA.
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