Establishment of plant tissue culture system and preliminary study on genetic transformation of Lvzhou No. 3

The calli derived from Lvzhou No. 3 (Arundo)were used as experimental material to induce adventitious buds and roots and conduct apot experiment. The calli were then used as receptors to examine the genetic transformation of Lvzhou No. 3. The results demonstrated that the most effective formula for adventitious bud induction was MS+1.0 mg·L-1 6-BA+0.1 mg·L-1 NAA, which exhibited an adventitious bud induction rate of 100%. The best formula for root induction was MS; the induction rate was 100%. Among nine different transplanting media, the rate of living seedling was 100% after being transplanted in peaty soil. The results showed that calli of Lvzhou No. 3(Arundo)that were precultured for 3 d, inoculated for 10 minutes, OD600=0.1, and cocultured for 1 d with 300 μmol·L-1AS are adapted for genetic transformation. The GUS expression rate might reach 1.5%.