Identification of the cyst nematode parasited on Achnatherum inebrians

To clarify the condition of cyst nematodes on Gramineae grasses that are infested with parasites, samples from roots and rhizosphere soil of Achnatherum inebrians (Hance) Keng were collected at Tianzhu alpine meadow steppe, Gansu, China in the middle of August, 2017, and a simple flooring separation method was used to separate nematodes from the roots and soil. The result indicated that the cereal cyst nematodes are found in the soil, and the white females are present on the roots of A. inebrians. The nematodes were initially identified as Heterodera avenae Wollenweber, 1924 based on morphological measuring and characteristics of the vulval fenestra, vulval slit, underbridge, and bullae. The ITS1 and 28S regions of ribosomal DNA of nematode populations were amplified and identified, and the phylogenetic trees were determined using the Maximum Likelihood method of MEGA 6.0. The ITS1 region of rDNA were genetically close to the population of H. avenae from China. The 28S rDNA region was genetically closed to the H. avenae population from the Czech Republic. Identification using morphological and molecular characteristics confirmed that the nematode found on A. inebrians was H. avenae. This is the first report to confirm that A. inebrians can parasitized by H. avenae. The study provides a theoretical basis for the prevention of A. inebrians.