Isolation, identification, and genomic sequencing analysis of an endophytic biocontrol strain of Pennisetum giganteum

This study used Pennisetum giganteum as an experimental material to isolate and purify strains with strong biological and defensive effects, which were then analyzed using genome sequencing. Strains with strong antibacterial effects were screened using plate confrontation. The strains were sequenced using the Illumina Miseq PE150, and the data were analyzed using the relevant software for genome analysis, gene prediction and functional annotation, and direct homologous cluster analysis. Streptomyces sp. JK7-2 was isolated from the bacteria of Pennisetum giganteum, and the genome of the strain was sequenced and analyzed. A total of 290 contiguous sequences were obtained. The whole genome size was about 8.4 Mb and the GC content was 73.17%. The sequence has been submitted to the NCBI database and BioProject under ID:PRJNA451251. In addition, it was predicted that 7 432 genes were encoded and several genes related to the streptomycin, penicillin, and tetracycline metabolic pathways were found. The results provide a theoretical basis for further study of the antibacterial mechanisms of the bacteria in the Pennisetum sp. and the genes related to the secondary metabolites of these bacteria.