Optimizing fermentation condition for Bacillus amyloliquefaciens JNC2 in flask

To investigate the best conditions of Fermentation liquid biomass of Bacillus amyloliquefaciens strain JNC2 and increase the antagonistic substance productivity against Colletotrichum gloeosporioides, the single factor experiments and orthogonal experiments were adopted to optimize the liquid fermentation medium and fermentation conditions. The results showed that the best optimize for JNC2 strain was 2% xylose, 0.4% peptone, 0.8% yeast powder, 0.6% ammonium chloride, and 0.2% magnesium sulfate. The optimum fermentation conditions were initial pH 6.0, medium capacity 60 mL in 250 mL triangular flask, 7% inoculum rate, fermentation temperature in 34 ℃, rotation speed in 200 r·min^–1 and fermentation time of 72 h. Inhibitory activity of strain JNC2 fermentation solution increased 32.61% under the optimum fermentation medium and culture conditions.