Evaluation of the nutritional value of mulberry leaves fed to Yunnan Yunling cattle

WANG Ningwei; HUANG Xianzhi; LIU Jianyong; HUANG Bizhi; SHEN Yihong

doi:10.11829/j.issn.1001-0629.2018-0623

Pratacultural Science > 2019 > 36(9): 2365-2373. > DOI: 10.11829/j.issn.1001-0629.2018-0623

WANG N W, HUANG X Z, LIU J Y, HUANG B Z, SHEN Y H. Evaluation of the nutritional value of mulberry leaves fed to Yunnan Yunling cattle. Pratacultural Science, 2019, 36(9): 2365-2373 . DOI: 10.11829/j.issn.1001-0629.2018-0623

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Evaluation of the nutritional value of mulberry leaves fed to Yunnan Yunling cattle

1.
State Key Laboratory of Silkworm Genome Biology, Chongqing 400715, China
2.
Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Chongqing 400715, China
3.
Grassland animal science research institute of Yunnan Province, Kunming 650212, Yunnan, China

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Corresponding author:
SHEN Yihong　E-mail: yhshen2006@163.com
Received Date: November 08, 2018
Accepted Date: March 05, 2019
Available Online: July 31, 2019
Published Date: August 31, 2019

Graphical Abstract

Abstract

Abstract

The content of routine nutrients, amino acids, and anti-nutrition factors present in mulberry leaves (Morus alba) was analysed using a chemical analysis method to evaluate its feed value. The degradation rates of the dry matter (DM), organic matter (OM), crude protein (CP), neutral detergent fibre (NDF), and acid detergent fibre (ADF) of mulberry leaves were determined using the nylon-bag technique. The results showed that the crude protein in mulberry leaves is 22.50% and thus it can be used as a protein source in feed. The effective degradation rates of the nutrients in mulberry leaves were CP 65.02%, ADF 48.39%, NDF 56.42%, DM 58.56%, and OM 59.42%, respectively. There were 17 kinds of amino acids detected in mulberry leaves; the essential amino acid (EAA)/toll amino acid (TAA) was 0.427 and the essential amino acid (EAA)/non-essential amino acid (NEAA) was 0.746. The closeness degree (μ) was only 0.524, and the score of ratio coefficient of amino acid (SRCAA) was only 65.02. The anti-nutritional factors in mulberry leaves were polysaccharides, tannins, saponins, flavonoids, phytic acid, DNJ, and oxalic acid. In conclusion, mulberry leaves showed high nutritional value and could be used as a protein feed. The imbalance of amino acids and the problem of anti-nutritional factors in feed necessitates adequate consideration.
- mulberry leaves,
- nutrition compositions,
- ruminal degradability,
- amino acid composition,
- anti-nutritional factors content

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References (40)

References

[1]	刘先珍, 朱建录, 刘晓华. 畜禽业新型饲料源:桑叶的营养价值及加工调制. 饲料工业, 2005(23): 46-47. doi: 10.3969/j.issn.1001-991X.2005.23.017 LIU X Z, ZHU J L, LIU X H. Nutritional value and processing of a new feed source: Mulberry leaf for livestock and poultry industry. Feed Industry, 2005(23): 46-47. doi: 10.3969/j.issn.1001-991X.2005.23.017
[2]	黄静, 邝哲师, 刘吉平, 邹宇晓, 赵祥杰, 沈维治, 廖森泰. 桑叶在动物饲料的应用研究现状与发展策略. 蚕业科学, 2014, 40(6): 1114-1121. HUANG J, KUANG Z S, LIU J P, ZOU Y X, ZHAO X J, SHEN W Z, LIAO S T. Current status and development strategy of using mulberry leaf as animal forage. Science of Sericulture, 2014, 40(6): 1114-1121.
[3]	李法见, 杨阳, 陈文燕, 林仕梅, 罗莉, 黄先智. 桑叶对罗非鱼生长性能、脂质代谢和肌肉品质的影响. 动物营养学报, 2014, 26(11): 3485-3492. doi: 10.3969/j.issn.1006-267x.2014.11.039 LI F J, YANG Y, CHEN W Y, LIN S M, LUO L, HUANG X Z. Effects of mulberry leaves on growth performance, lipid metabolism and muscle quality of tilapia. Journal of Animal Nutrition, 2014, 26(11): 3485-3492. doi: 10.3969/j.issn.1006-267x.2014.11.039
[4]	马恒甲, 刘新轶, 谢楠, 刘凯, 潘彬斌, 黄辉. 草鱼饲料中桑叶粉的应用初探. 科学养鱼, 2013(5): 79-80. MA H J, LIU X Y, XIE N, LIU K, PAN B B, HUANG H. Preliminary study on application of mulberry leaf powder in grass carp feed. Scientific Fish Farming, 2013(5): 79-80.
[5]	孟留伟, 周逸斌, 黄凌霞. 桑叶的饲用价值及其在动物生产中的应用和研究现状. 蚕桑通报, 2016, 47(3): 11-16. doi: 10.3969/j.issn.0258-4069.2016.03.003 MENG L W, ZHOU Y B, HUANG L X. Feeding value of mulberry leaves and current status of research and application in animal production. Bulletin of Sericulture, 2016, 47(3): 11-16. doi: 10.3969/j.issn.0258-4069.2016.03.003
[6]	张丽英. 饲料分析及饲料质量检测技术. 2版. 北京: 中国农业大学出版社, 2003: 45-79. ZHANG L Y. Feed Analysis and Feed Quality Inspect Technology. 2nd edition. Beijing: China Agricultural University Press, 2003: 45-79.
[7]	VAN SOEST P J. Carbohydrate methodology, metabolism, and nutritional implications in dairy cattle: Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 1991, 74(10): 3583-3597. doi: 10.3168/jds.S0022-0302(91)78551-2
[8]	黄健, 闵星, 陆春燕. 主要桑品种桑叶黄酮化合物含量的比较分析. 江苏蚕业, 2013, 35(2): 12-14. doi: 10.3969/j.issn.1003-9848.2013.02.005 HUANG J, MIN X, LU C Y. Comparative analysis of flavonoids content in main mulberry varieties. Jiangsu Sericulture, 2013, 35(2): 12-14. doi: 10.3969/j.issn.1003-9848.2013.02.005
[9]	辛文. 桑叶多糖的提取及其生物活性的研究. 成都: 西华大学硕士学位论文, 2014. XIN W. Research of extraction and biological activity of polysaccharides from mulberry leaves. Master Thesis. Chengdu: Xihua University, 2014.
[10]	蒲俊松. 桑叶生物碱的检测提取及含量分析. 重庆: 西南大学硕士学位论文, 2016. PU J S. Determination, extraction and concentration analysis of alkaloid in mulberry leaves. Master Thesis. Chongqing: Southwest University, 2016.
[11]	杨琼琼.柿木皮单宁提取纯化及抗氧化活性研究. 南宁: 广西大学硕士学位论文, 2016. YANG Q Q. Study on extraction, purification and antioxidant activity of tannin from Persimmon veneer. Master Thesis. Nanning: Guangxi University, 2016.
[12]	范东翠, 俞彦波, 胡建, 周才琼. 不同加工处理对卡亚草酸含量影响. 食品科技, 2011, 36(4): 71-73. FAN D C, YU Y B, HU J, ZHOU C Q. Effect on Chaya (Cnidoscolus spp.) leaves oxalic acid content in treatment processing. Food Science and Technology, 2011, 36(4): 71-73.
[13]	郝红英, 周芳, 周彩荣, 叶文见. 分光光度法测定植酸含量. 安徽农业科学, 2014, 42(7): 1907-1975. doi: 10.3969/j.issn.0517-6611.2014.07.002 HAO H Y, ZHOU F, ZHOU C R, YE W J. Determination on phytic acid of by spectrophotometry. Journal of Anhui Agricultural Sciences, 2014, 42(7): 1907-1975. doi: 10.3969/j.issn.0517-6611.2014.07.002
[14]	袁柱, 孙彦, 陆继肖, 冯子蓉. 不同品种苜蓿青贮总皂苷含量变化的研究. 草地学报, 2015, 23(1): 194-199. doi: 10.11733/j.issn.1007-0435.2015.01.030 YU Z, SUN Y, LU J X, FENG Z R. Study on the total saponin contents of different alfalfa silages. Acta Agrectir Sinica, 2015, 23(1): 194-199. doi: 10.11733/j.issn.1007-0435.2015.01.030
[15]	李茂, 字学娟, 周汉林, 刘国道, 侯冠彧, 徐铁山. 海南省部分热带灌木饲用价值评定. 动物营养学报, 2012, 24(1): 85-94. doi: 10.3969/j.issn.1006-267x.2012.01.013 LI M, ZI X J, ZHOU H L, LIU G D, HOU G Y, XU T S. Feeding value assessment of some tropical shrubs in Hainan Province. Chinese Journal of Animal Nutrition, 2012, 24(1): 85-94. doi: 10.3969/j.issn.1006-267x.2012.01.013
[16]	王慧娟. 羊常用粗饲料营养价值评定. 杨凌: 西北农林科技大学硕士学位论文, 2017. WANG H J. Evaluation of nutritive value of common forage in sheep. Master Thesis. Yangling: Northwest A&F University, 2017.
[17]	ΦRSKOV E R, MCDONALD I. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. Journal of Agricultural Science, 1979, 92(2): 499-503.
[18]	杨有泉, 姜传京, 应朝阳, 陆烝, 陈敏, 邓素芳. 红萍叶蛋白氨基酸成分分析及营养价值评价研究. 食品工业, 2017, 38(5): 285-289. YANG Y Q, JIANG C J, YING Z Y, LU Z, CHEN M, DENG S F. LPC of azolla amino acid composition analysis and nutritional value evaluation research. The Food Industry, 2017, 38(5): 285-289.
[19]	PELLETT P L. Nutrition assessment of protein foods//YOUNG V R. Essential Amino Acid Pattern for Evaluating Protein Nutritional Value. Beijing: People' s Medical Publishing House, 1984:55-58.
[20]	刘洁, 刁其玉, 赵一广, 姜成钢, 邓凯东, 李艳玲, 屠焰. 肉用绵羊饲料养分消化率和有效能预测模型的研究. 畜牧兽医学报, 2012, 43(8): 1230-1238. LIU J, DIAO Q Y, ZHAO Y G, JIANG C G, DENG K D, LI Y L, TU Y. Prediction of nutrient digestibility and energy concentrations using chemical composition in meat sheep feeds. Acta Veterinaria et Zootechnica Sinica, 2012, 43(8): 1230-1238.
[21]	霍子建,安永胜,崔小波. 草食家畜几种常用饲料的分类及特点. 养殖技术顾问, 2014(11): 68. HUO Z J, AN Y S, CUI X B. Classification and characteristics of several common feeds for herbivorous livestock. Technical Advisor for Animal Husbandry, 2014(11): 68.
[22]	高月娥, 蔡明, 黄必志, 王馨, 刘彦培, 张继才, 刘建勇. 桑叶在肉牛瘤胃中的降解特性研究. 山地农业生物学报, 2017, 36(4): 80-84. GAO Y E, CAI M, HUANG B Z, WANG X, LIU Y P, ZHANG J C, LIU J Y. Study on degradation characteristic of mulberry leaf in rumen of beef cattle. Journal of Mountain Agriculture and Biology, 2017, 36(4): 80-84.
[23]	李茂, 字学娟, 白昌军, 周汉林, 刘国道. 不同生长高度王草瘤胃降解特性研究. 畜牧兽医学报, 2015, 46(10): 1806-1815. LI M, ZI X J, BAI C J, ZHOU H L, LIU G D. Rumen degradation characteristics of Kinggrass at different plant heights. Acta Veterinaria et Zootechnica Sinica, 2015, 46(10): 1806-1815.
[24]	裘燕.奶牛常用粗饲料的营养成分及其瘤胃降解率与体外发酵特性. 北京: 中国农业大学硕士学位论文, 2013. QIU Y. Nutritional composition, rumen degradation rate and in vitro fermentation characteristics of common rough feed for dairy cows. Master Thesis. Beijing: China Agricultural University, 2013.
[25]	陈艳, 张晓明, 王之盛, 彭全辉, 邹华围. 6种肉牛常用粗饲料瘤胃降解特性和瘤胃非降解蛋白质的小肠消化率. 动物营养学报, 2014, 26(8): 2145-2154. doi: 10.3969/j.issn.1006-267x.2014.08.016 CHEN Y, ZHANG X M, WANG Z S, PENG Q H, ZOU H W. Degradation characteristics of rumen and intestinal digestibility of non-degraded proteins in the rumen of 6 kinds of commonly used feedstuffs for beef cattle. Chinese Journal of Animal Nutrition, 2014, 26(8): 2145-2154. doi: 10.3969/j.issn.1006-267x.2014.08.016
[26]	李振.秦川肉牛常用饲料瘤胃降解特性研究.杨凌: 西北农林科技大学硕士学位论文, 2016. LI Z.Ruman degradation characteristics of commonly used feed for Qinchuan beef cattle. Master Thesis. Yangling: Northwest A&F University, 2016.
[27]	崔延春, 王曼玲, 吴端钦, 夏新界. 巨型稻与超级稻秸秆营养成分及饲用价值. 草业科学, 2018, 35(9): 2285-2290. doi: 10.11829/j.issn.1001-0629.2017-0660 CUI Y C, WANG M L, WU D Q, XIA X J. Nutritive components and feed value of giant rice and super hybrid rice straw. Pratacultural Science, 2018, 35(9): 2285-2290. doi: 10.11829/j.issn.1001-0629.2017-0660
[28]	杨静.饲料桑粉的营养价值评定及在生长育肥猪日粮中的应用研究.保定: 河北农业大学硕士学位论文, 2014. YANG J. Study on the nutritional value of forage mulberry powder and research in dietary of finishing pigs. Master Thesis. Baoding: Agricultural University of Hebei, 2014.
[29]	李雪影, 陆宁, 张晶, 徐辉, 郭秉政. 薇菜干氨基酸组成分析与营养价值评价. 食品与机械, 2015(6): 28-32. LI X Y, LU N, ZHANG J, XU H, GUO B Z. Analysis of amino acids composition and nutritional value evaluation of dried osmund. Food & Machinery, 2015(6): 28-32.
[30]	王芳, 乔璐, 张庆庆, 沈斌. 桑叶蛋白氨基酸组成分析及营养价值评价. 食品科学, 2015, 36(1): 225-228. doi: 10.7506/spkx1002-6630-201501043 WANG F, QIAO L, ZHANG Q Q, SHEN B. Amino acid composition and nutritional evaluation of mulberry leaves. Food Science, 2015, 36(1): 225-228. doi: 10.7506/spkx1002-6630-201501043
[31]	范东翠.卡亚抗营养因子及其失活条件研究.重庆: 西南大学硕士学位论文, 2010. FAN D C. Antinutritional factor analysis and effect of treatment processing on them in Chaya (Cnidoscolus spp.) leaves. Master Thesis. Chongqing: Southwest University, 2010.
[32]	张鸿, 王晴芳, 孙波, 熊超, 宋忠旭. 桑树中α-葡萄糖苷酶抑制剂对桑饲料功能的影响. 湖北农业科学, 2013, 52(19): 4737-4739. ZHANG H, WANG Q F, SUN B, XIONG C, SONG Z X. Effect of α-glucosidase inhibitors in Morus on it feed function. Hubei Agricultural Sciences, 2013, 52(19): 4737-4739.
[33]	穆志新, 田翔, 师颖. 高粱中单宁含量的检测方法分析. 山西农业科学, 2014, 42(6): 551-553. doi: 10.3969/j.issn.1002-2481.2014.06.04 MU Z X, TIAN X, SHI Y. Determination method of tannin content in sorghum. Shanxi Agricultural Sciences, 2014, 42(6): 551-553. doi: 10.3969/j.issn.1002-2481.2014.06.04
[34]	吴德锦.常见菌草菌糠营养特性及抗营养因子研究.福州: 福建农林大学硕士学位论文, 2014. WU D J. Research for nutritional properties and antinutritional factor of the common JUNCAO and spent mushroom substrates. Master Thesis. Fuzhou: Fujian Agriculture and Forestry University. 2014.
[35]	李元恒, 金龙, 韩国栋, 王正文, 赵萌莉, 王玉玺. 植物单宁在反刍动物营养和健康养殖作用中的研究进展. 草地学报, 2013, 21(6): 1043-1051. doi: 10.11733/j.issn.1007-0435.2013.06.002 LI Y H, JIN L, HAN G D, WANG Z W, ZHAO M L, WANG Y X. Effects of plant tannin on the nutrition and healthy production of ruminant: A review. Acta Agrestia Sinica, 2013, 21(6): 1043-1051. doi: 10.11733/j.issn.1007-0435.2013.06.002
[36]	DONNELLY E D, ANTHONY W B. Relationship of tannin, dry matter digestibility and crude protein in Sericea lespedeza. Crop Science, 1969, 9(3): 361-362.
[37]	CHIKWATI E M, VENOLD F F, PENN M H, ROHLOFF J, REFSTIE S, GUTTVIK A, HILLESTAD M, KROGDAHI A. Interaction of soyasaponins with plant ingredients in diets for Atlantic salmon, Salmo salar L. British Journal of Nutrition, 2012, 107(11): 1570-1590. doi: 10.1017/S0007114511004892
[38]	魏伯平, 张咏梅, 曹致中. 9种豆科牧草总黄酮和总皂苷积累规律的研究. 草地学报, 2012, 20(1): 88-95. doi: 10.11733/j.issn.1007-0435.2012.01.015 WEI B P, ZHANG Y M, CAO Z Z. Accumulation patterns of total flavonoids and saponins in nine legume species. Acta Agrectir Sinic, 2012, 20(1): 88-95. doi: 10.11733/j.issn.1007-0435.2012.01.015
[39]	尹尧. 饲料中植酸的抗营养作用. 畜牧兽医科技信息, 2015(5): 45. doi: 10.3969/J.ISSN.1671-6027.2015.05.033 YIN Y. The antinutritive action of phytic acid in feed. Chinese Journal of Animal Husbandry and Veterinary Medicine, 2015(5): 45. doi: 10.3969/J.ISSN.1671-6027.2015.05.033
[40]	HANG D T, HAI P V, HAI V V, DUC N L, MINH T L. Oxalate content of taro leaves grown in central Vietnam. Foods, 2017, 6(1): 2-. doi: 10.3390/foods6010002

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