Welcome Pratacultural Science,Today is
XU L, HAN J T, FENG G Y, LIU Y N, HUANG L K, ZHANG X Q. Study of factors affecting callus induction from mature seeds of orchardgrass. Pratacultural Science, 2020, 37(1): 87-97.. DOI: 10.11829/j.issn.1001-0629.2019-0195
Citation: XU L, HAN J T, FENG G Y, LIU Y N, HUANG L K, ZHANG X Q. Study of factors affecting callus induction from mature seeds of orchardgrass. Pratacultural Science, 2020, 37(1): 87-97.. DOI: 10.11829/j.issn.1001-0629.2019-0195

Study of factors affecting callus induction from mature seeds of orchardgrass

  • To establish an efficient regeneration system for orchardgrass, mature seeds were studied to determine the effects of seed pretreatment, variety selection, medium selection, and hormone ratio on the process of callus induction. The results showed a statistically significant (P < 0.05) effect of NaClO concentration on callus induction rate among the three pretreatment conditions. The cultivar ‘Anba’ had the strongest callus induction ability among the ten cultivars. Callus induction ability and morphological development were related to the medium used: Schenk & Hildebrandt medium (SH), Murashige & Skoog medium (MS), and Linsmaier & Skoog medium (LS) (all resulted in higher callus induction rates; of these, SH had the best induction effect and yellowish or yellow, dry, and crisp calli were obtained). It was thought that 2,4-D with 30 μmol·L–1 dicamba would promote callus induction; in addition, the combination of 2,4-D and a lower concentration 6-KT was also beneficial for callus induction. In conclusion, the seeds of ‘Anba’ were first placed at 4 ℃ for 24 h after soaking, then treated with 75% alcohol for 5 min, and sterilized with 1.1% NaClO for 30 min. The optimum medium for callus induction was SH + 2.0 mg·L–1 CPA + 0.1 mg·L–1 6-KT + 30 μmol·L–1 dicamba + 0.5 mg·L–1 2,4-D. With this medium and hormone combination, the callus induction efficiency was high and embryonic calli were more readily induced. Thus, an efficient and stable preliminary tissue culture and regeneration system was established for orchardgrass.
  • loading

Catalog

    Turn off MathJax
    Article Contents

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return