Globodera artemisiae, a new cyst nematode record in Gansu, China

In 2018−2019, a cyst nematode population was isolated from the rhizosphere of Artemisia argyi on an alpine grassland in Tianzhu County, Gansu Province, China. The morphological characteristics of this nematode population were: brown spherical cysts, a prominent neck, generally circumfenestrate, with no vulval cone, underbridge, or bullae. Second-stage juveniles were vermiform, with inconspicuous head constriction, and a strong stylet. The tail was conical and sharply pointed. The ITS-rDNA and 28S-rRNA D2-D3 regions of the population were amplified, from which 948 and 1 015 bp fragments were obtained, respectively. After sequencing, cluster analysis showed that the population was clustered as Globodera artemisiae, with a confidence of over 97%. This population was identified as G. artemisiae based on its morphological and molecular characteristics. This is the first report of the genus Globodera and a new species record in Gansu Province.