Genetic diversity and population structure analysis of Paspalum vaginatum based on SRAP markers

In order to explore Paspalum vaginatum, the genetic diversity of 49 P. vaginatum germplasm resources from different countries was analyzed using SRAP markers, and the population structure and genetic relationships among the germplasm was discussed. Twenty pairs of SRAP primers with stable amplification and good polymorphism were screened out from 100 pairs of primers, and 169 bands were obtained, among which 118 were polymorphic; the percentage of polymorphic sites was 69.8%. The genetic similarity coefficient among 49 P. vaginatum germplasms was 0.52～0.88, with an average value of 0.67, indicating that there were significant genetic differences among the tested germplasms. 49 Paspalum germplasms were divided into two categories by the UPGMA cluster analysis, principal component analysis, and population structure analysis. Forty-four materials showed a more consistent classification under the three methods, and five materials showed a certain degree of variation, indicating that the genetic relationship of the germplasm was stable and could be used in breeding.