Lycityum ruthenicum leave membrane permeability and protection system response to the blowing of net-wind and wind-sand flow with different time durations

In this study, we investigated the variations in malondialdehyde (MDA) content, membrane permeability, protective enzyme activity, and osmoregulation substance content in two-year-old potted Lycium ruthenicum leaves. The net-wind and wind-sand flow conditions were simulated in a wind tunnel for 0, 10, 20, and 30 min with a wind speed of 12 m·s⁻¹ and 63.28 g·(cm·min)⁻¹ wind-sand flow intensity blowing stress, in order to reveal Lycium ruthenicum adaptability and response mechanism to net wind and wind-sand flow stress. The results showed that the net-wind treatment first decreased then increased the MDA content; gradually decreased the soluble protein (SP) content; led to an increasing trend in membrane permeability, peroxidase (POD), soluble sugar (SS), and  proline (Pro) contents; and first increased then decreased the catalase (CAT) and superoxide dismutase (SOD) hydrogen peroxide enzyme activities. The wind-sand flow treatment resulted in a first increasing then decreasing MDA content; POD, CAT, Pro, and SS trend, although they were higher than CK, membrane permeability increase; SP content decrease; and a first decreasing then increasing SOD activity. The results indicated that Lycium ruthenicum leaves were damaged to a certain extent under the blowing of the net wind and wind-sand flow, and both enhanced the permeation regulation through the Pro and SS content accumulation, improving the ability of the plants to resist wind and sand. Under net-wind stress, POD activity plays a protective role, whereas under wind-sand flow stress, SOD, POD, and CAT activities play a coordinated protective role in reducing cell membrane damage.