Cloning and expression of the AcDREB2 transcription-factor gene in Atriplex canescens

Dehydration-responsive element-binding proteins (DREB) play an important role in plant responses to salt and drought stress. To investigate the function of DREB in Atriplex canescens and their application prospects, a pair of degenerate primers was designed, and the DREB transcription-factor coding-gene AcDREB2 was cloned via PCR (polymerase chain reaction) amplification and the RACE (Remote Analysis Computation for gene Expression data) method. The full-length cDNA of AcDREB2 was 867 bp, encoding 242 amino acids. The BLAST and homology matrix results showed that AcDREB2 was highly homologous with the DREBs of other plants. The expression patterns of AcDREB2 were analyzed via RT-PCR. AcDREB2 was highly expressed in the leaves of A. canescens, and its expression was rapidly induced by NaCl and osmotic stress, indicating that AcDREB is involved in the A. canescens stress response.