Evaluation of Pb resistance in seedlings of four common aquatic <i>Iris</i> species<italic/>

BO Wei; WANG Song; WANG Jin; KANG Hongmei; LIU Chenbin

doi:10.11829/j.issn.1001-0629.2021-0561

Pratacultural Science > 2022 > 39(8): 1571-1578. > DOI: 10.11829/j.issn.1001-0629.2021-0561

BO W, WANG S, WANG J, KANG H M, LIU C B. Evaluation of Pb resistance in seedlings of four common aquatic species. Pratacultural Science, 2022, 39(8): 1571-1578 . DOI: 10.11829/j.issn.1001-0629.2021-0561

Citation:

PDF (837 KB)

Evaluation of Pb resistance in seedlings of four common aquatic Iris species

Taking College of Horticulture of Shanxi Agricultural University, Taiyuan 030031, Shanxi, China

More Information

Corresponding author:
LIU Chenbin　E-mail: lcb_197811@163.com
Received Date: September 14, 2021
Available Online: June 20, 2022
Published Date: August 14, 2022

Graphical Abstract

Abstract

Abstract

In this study, we assessed and compared the changes in physiological, growth, and stress indices in the seedlings of four common aquatic Iris species (i.e., I. sibirica, I. ensata var. hortensis, I. pseudacorus, I. ensata). The nutrient solution culture method was used and seedlings were exposed to 1 200 mg·L⁻¹ of lead (Pb) stress for 20 d. The results showed large differences in the degree of Pb-induced damage to the different Iris species. The malondialdehyde (MDA) content stress index had a range of 125.39%～748.00%; the soluble protein (SP) and proline (pro) content stress indices had ranges of 104.77%～143.99% and 77.49%～340.20%, respectively, while the superoxide dismutase (SOD) and catalase (CAT) activity stress indices had ranges of 57.61%～106.15% and 26.85%～97.11%, respectively. Furthermore, the degree of Pb-induced damage to Iris ensata var. hortensis was mild, while the Pb enrichment, bioconcentration factor, and transport coefficient of Iris ensata var. hortensis was the largest. The principal component analysis classified twelve indices into three groups, namely the extent of injury, Pb-resistant ability, and growth status; moreover, the contribution of the first principal component was relatively high (55.02%). The contributions of twelve Pb resistance indices, including leaf length (LL), leaf width (LW), root fresh weight (RFW), stem and leaf fresh weight (SFW), and root dry weight (RDW), were higher in the first principal component, indicating that these indices may reflect the Pb resistance found in aquatic Iris species. Differences in the comprehensive score and subordinate function value of the twelve Pb resistance indices of each Iris species were large, with I. ensata var. hortensis and I. sibirica displaying higher values. Ultimately, the ranking of Pb tolerance in the four aquatic Iris species was: I. ensata var. hortensis > I. sibirica > I. pseudacorus > I. ensata. Finally, through this comprehensive analysis, we suggest that the Pb resistance, Pb enrichment, and transport coefficient of I. ensata var. hortensis and I. sibirica are superior to the other two species assessed here, suggesting that these two Iris species could be used for the phytoremediation of Pb-polluted water.
- Pb stress,
- principal component analysis,
- subordinate function analysis,
- Pb enrichment,
- physiological indices

FullText(HTML)

References (29)

References

[1]	NEMATI K, BAKAR N K A, ABAS M R, SOBHANZADEH E. Speciation of heavy metals by modified BCR sequential extraction procedure in different depths of sediments from Sungai Buloh, Selangor, Malaysia. Journal of Hazardous Materials, 2011, 192(1): 402-410.
[2]	MCLAUGHIIN M J, PARKER D R, CLARKE J M. Metals and micronutrients-food safety issues. Field Crops Research, 1999, 60: 143-163. doi: 10.1016/S0378-4290(98)00137-3
[3]	张思悦. 黄葛树对土壤Pb、Cd污染的修复潜力研究. 重庆: 西南大学硕士学位论文, 2018. ZHANG S Y. Study on the phytoremedial potential of Ficus virens Aiton in soil contaminated by Pb and Cd. Master Thesis. Chongqing: Southwest University, 2018.
[4]	葛海香, 邢万堂. 铅胁迫对垂盆草生长与生理指标影响的初步研究. 特种经济动植物, 2020, 23(12): 25-27. doi: 10.3969/j.issn.1001-4713.2020.12.013 GE H X, XING W T. Study of effects of lead stress on growth and physiology of Sedum sarmentosum. Special Economic Animal and Plant, 2020, 23(12): 25-27. doi: 10.3969/j.issn.1001-4713.2020.12.013
[5]	陈晶晶, 王英哲, 金艳, 周仂, 徐博. 铅胁迫对14个紫花苜蓿杂交组合生理生化指标的影响. 草地学报, 2018, 26(5): 1277-1282. CHEN J J, WANG Y Z, JIN Y, ZHOU L, XU B. Physiological changes and tolerance evaluation of different alfalfa hybrid combination under lead stress treatments. Acta Agrestia Sinica, 2018, 26(5): 1277-1282.
[6]	赵利清, 彭向永, 刘俊祥, 毛金梅, 孙振元. GSH对铅胁迫下多年生黑麦草幼苗抗氧化系统的调控. 草地学报, 2020, 28(6): 1527-1534. ZHAO L Q, PENG X Y, LIU J X, MAO J M, SUN Z Y. Effects of glutathione (GSH) on antioxidant defense system of Lolium perenne seedlings under lead stress. Acta Agrestia Sinica, 2020, 28(6): 1527-1534.
[7]	安琪. 深圳洪湖公园水生植物的配置. 河北林果研究, 2011, 26(2): 214-216. doi: 10.3969/j.issn.1007-4961.2011.02.027 AN Q. The configuration of aquatic plants in Shenzhen Honghu Park. Hebei Journal of Forestry and Orchard Research, 2011, 26(2): 214-216. doi: 10.3969/j.issn.1007-4961.2011.02.027
[8]	施旭丽, 朱安超, 陈发棣, 房伟民, 管志勇, 陈素梅. 17个菊花品种幼苗的耐镉性评价. 植物资源与环境学报, 2015, 24(3): 50-59. doi: 10.3969/j.issn.1674-7895.2015.03.07 SHI X L, ZHU A C, CHEN F D, FANG W M, GUAN Z Y, CHEN S M. Evaluation on Cd resistance of seedlings of 17 cultivars of Dendranthema morifolium. Journal of Plant Resources and Environment, 2015, 24(3): 50-59. doi: 10.3969/j.issn.1674-7895.2015.03.07
[9]	WENG M, CUI L, LIU F, MIN Z, DENG X. Effects of drought stress on antioxidant enzymes in seedlings of different wheat genotypes. Pakistan Journal of Botany, 2015, 47(1): 49-56.
[10]	ZHANG F Q, WANG Y S, LOU Z P, DONG J D. Effect of heavy metal stress on antioxidative enzymes and lipidperoxidation in leaves and roots of two mangrove plant seedlings (Kandeliacandel and Bruguiera gymnorrhiza). Chemosphere, 2007, 67(1): 44-50. doi: 10.1016/j.chemosphere.2006.10.007
[11]	陈建勋. 植物生理学实验指导. 广州: 华南理工大学出版社, 2011: 72-73. CHEN J X. Experimental Guidance of Plant Physiology. Guangzhou: South China University of Technology Press, 2011: 72-73.
[12]	BATES L, WALDREN R, TEARE D. Rapid determination of free proline for water-stress studies. Plant and Soil, 1973, 39: 205-207. doi: 10.1007/BF00018060
[13]	FU L, DING Z, HAN B, HU W, LI Y, ZHANG J. Physiological investigation and transcriptome analysis of polyethylene glycol (PEG)-induced dehydration stress in cassava. International Journal of Molecular Sciences, 2016, 17(3): 283-301. doi: 10.3390/ijms17030283
[14]	田松青, 朱旭东, 原海燕, 黄苏珍. 铅胁迫下路易斯安那鸢尾铅吸收量和非蛋白巯基的变化. 北方园艺, 2015(21): 77-82. doi: 10.11937/bfyy.201521022 TIAN S Q, ZHU X D, YUAN H Y, HUANG S Z. Effect of Pb stress on Pb accumulation and non-protein thiol content in Louisiana Iris. Northern Horticulture, 2015(21): 77-82. doi: 10.11937/bfyy.201521022
[15]	潘杰, 来守军, 黄怡民, 何立平, 余顺慧. 铅胁迫对2种三峡库区消落带适生植物生长及铅积累的影响. 江苏农业科学, 2014, 42(6): 332-335. doi: 10.3969/j.issn.1002-1302.2014.06.116 PAN J, LAI S J, HUANG Y M, HE L P, YU S H. Effects of lead stress on growth and lead accumulation of two suitable plants in the fluctuating zone of the three gorges reservoir area. Jiangsu Agricultural Sciences, 2014, 42(6): 332-335. doi: 10.3969/j.issn.1002-1302.2014.06.116
[16]	原海燕, 郭智, 黄苏珍. Pb污染对马蔺生长、体内重金属元素积累以及叶绿体超微结构的影响. 生态学报, 2011, 31(12): 3350-3357. YUAN H Y, GUO Z, HUANG S Z. Effects of Pb on growth, heavy metals accumulation and chloroplast ultrastructure of Iris lactea var. Chinensis. Acta Ecologica Sinica, 2011, 31(12): 3350-3357.
[17]	王鸿燕, 黄苏珍, 原海燕, 陈晓萱, 马万荣. Pb和Cd单一及复合胁迫条件下溪荪(Iris sanguinea)生长及金属离子积累特征分析. 植物资源与环境学报, 2011, 20(3): 24-28. doi: 10.3969/j.issn.1674-7895.2011.03.004 WANG H Y, HUANG S Z, YUAN H Y, CHEN X X, MA W R. Characteristics analyses of growth and metal ions accumulation of Iris sanguinea under single and combination stresses of Pb and Cd. Journal of Plant Resources and Environment, 2011, 20(3): 24-28. doi: 10.3969/j.issn.1674-7895.2011.03.004
[18]	PANDA S K. Chromium-mediated oxidative stress and ultrastructural changes in root cells of developing rice seedlings. Journal of Plant Physiology, 2007, 164(11): 1419-1428. doi: 10.1016/j.jplph.2007.01.012
[19]	粟春青, 蒋霞, 亢亚超, 赵苹羽, 王凌晖, 滕维超. 金花茶幼苗对铅胁迫的生长生理响应. 森林与环境学报, 2019, 39(5): 467-474. LI C Q, JIANG X, KANG Y C, ZHAO P Y, WANG L H, TENG W C. Growth and physiological response of Camellia petelotii seedlings to Pb stress. Journal of Forest and Environment, 2019, 39(5): 467-474.
[20]	IQBAL M M, MURTAZA G, SAQIB Z A, AHMAD R. Growth and physiological responses of two rice varieties to applied lead in normal and salt-affected soils. International Journal of Agriculture and Biology, 2015, 17(5): 901-910. doi: 10.17957/IJAB/15.0026
[21]	刘敏, 厉悦, 汲文宪, 梁艳, 张志, 陈阳. 五种黄色系菊科植物幼苗对干旱胁迫的生理响应及抗旱性评价. 北方园艺, 2016(11): 61-67. LIU M, LI Y, JI W X, LIANG Y, ZHANG Z, CHEN Y. Physiological response of seedlings of five yellow compositae species to drought stress and their drought resistance. Northern Horticulture, 2016(11): 61-67.
[22]	杨小琴, 赵运林, 孙玉珍. 凤仙花生物量及抗氧化酶系统对重金属铅胁迫的生理响应. 安徽农业科学, 2008, 36(9): 3526-3528. doi: 10.3969/j.issn.0517-6611.2008.09.024 YANG X Q, ZHAO Y L, SUN Y Z. Study on the physiological response of the biomass and antioxidant enzyme system of Impatiens balsamina L. to heavy metal Pb stress. Journal of Anhui Agricultural Sciences, 2008, 36(9): 3526-3528. doi: 10.3969/j.issn.0517-6611.2008.09.024
[23]	韩露, 张小平, 刘必融. 香根草对重金属铅离子的胁迫反应研究. 应用生态学报, 2005, 16 (11): 2178-2181. HAN L, ZHANG X P, LIU B R. Response of Vetiveria zizanioides to Pb^{2 +} stress. Chinese Journal of Applied Ecology, 2005, 16 (11): 2178-2181.
[24]	徐延浩, 侯泽豪, 张文英, 刘晓雪, 杨飞. 5-氮杂胞苷和丁酸钠对大麦苗期耐湿性的影响. 广东农业科学, 2015, 42(24): 26-30. doi: 10.3969/j.issn.1004-874X.2015.24.007 XU Y H, HOU Z H, ZHANG W Y, LIU X X, YANG F. Effects of 5-azacytidine and sodium butyrate on waterlogging tolerance during seeding stage in barley. Guangdong Agricultural Sciences, 2015, 42(24): 26-30. doi: 10.3969/j.issn.1004-874X.2015.24.007
[25]	刘慧芹, 韩巨才, 刘慧平, 康欣然. 铅梯度胁迫对多年生黑麦草幼苗生理生化特性影响. 草业学报, 2012, 21(6): 57-63. doi: 10.11686/cyxb20120608 LIU H Q, HAN J C, LIU H P, KANG X R. Influence of lead gradient stress on the physiological and biochemical characteristics of perennial ryegrass (Lolium perenne) seedlings. Acta Prataculturae Sinica, 2012, 21(6): 57-63. doi: 10.11686/cyxb20120608
[26]	王芳, 李永生, 王汉宁, 彭云玲, 方永丰, 王威, 马原忠. 钙对铅胁迫下玉米幼苗生长及生理特性的影响. 水土保持学报, 2016, 30(3): 202-207. WANG F, LI Y S, WANG H N, PENG Y L, FANG Y F, WANG W, MA Y Z. Effect of calcium on growth and physiological characteristics of maize seedling under lead stress. Journal of Soil and Water Conservation, 2016, 30(3): 202-207.
[27]	毛雪飞, 何金娇, 韩忠康, 武峰峰. 铅胁迫对金银花生长、生理及积累特性的影响. 东北农业科学, 2019, 44(5): 69-75. MAO X F, HE J J, HAN Z K, WU F F. Effects of lead stress on growth and physiological metabolism and accumulation characteristics of Lonicera Japonica. Journal of Northeast Agricultural Sciences, 2019, 44(5): 69-75.
[28]	孙静, 曾俊, 王银杰, 陈发棣, 房伟民, 陈素梅. 20个切花菊品种抗旱性评价与筛选. 南京农业大学学报, 2013, 36(1): 24-28. doi: 10.7685/j.issn.1000-2030.2013.01.005 SUN J, ZENG J, WANG Y J, CHEN F L, FANG W M, CHEN S M. Evaluation and screening of drought stress tolerance in 20 cut chrysanthemum varieties. Journal of Nanjing Agricultural University, 2013, 36(1): 24-28. doi: 10.7685/j.issn.1000-2030.2013.01.005
[29]	BO W, FU B C, QIN G J, XING G M, WANG Y G. Evaluation of drought resistance in Iris germanica L. based on subordination function and principal component analysis. Emirates Journal of Food and Agriculture, 2017, 29(10): 770-778.

Cited By

Get Citation

PDF

XML

Article views (1861) PDF downloads (14)

Turn off MathJax

Article Contents

Abstract

References

Evaluation of Pb resistance in seedlings of four common aquatic Iris species

Abstract

References

Catalog

Export File

Citation

Format

Content