Effect of different root separation methods on kin recognition and selection of <i>Stylosanthes guianensis</i> ‘Reyan No. 5’

ZHAO Yuanchen; LI Xuefeng; WANG Liyuan; XU Chao

doi:10.11829/j.issn.1001-0629.2022-0223

Pratacultural Science > 2023 > 40(2): 491-501. > DOI: 10.11829/j.issn.1001-0629.2022-0223

ZHAO Y C, LI X F, WANG L Y, XU C. Effect of different root separation methods on kin recognition and selection of ‘Reyan No. 5’. Pratacultural Science, 2023, 40(2): 491-501 . DOI: 10.11829/j.issn.1001-0629.2022-0223

Citation:

PDF (2324 KB)

Effect of different root separation methods on kin recognition and selection of Stylosanthes guianensis ‘Reyan No. 5’

Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants, Ministry of Education / College of forestry, Hainan University, Haikou 570228, Hainan, China

More Information

Corresponding author:
LI Xuefeng　E-mail: xfli0922@163.com
Received Date: March 22, 2022
Accepted Date: June 05, 2022
Available Online: December 11, 2022
Published Date: February 14, 2023

Graphical Abstract

Abstract

Abstract

This study assessed the effects of different root separation methods on kin recognition and selection of Stylosanthes guianensis ‘Reyan No. 5’. Three root separation methods were used: (i) no separation, (ii) nylon mesh, and (iii) plastic film. ‘Reyan No. 5’ was grown alone and separately with three cultivars ‘Reyan No. 2’, ‘Reyan No. 21’, and ‘Reyan No. 24’, in pot experiments. Changes in the morphological characteristics (plant height, number of branches, root length, and number of lateral roots) and biomass allocation (aboveground biomass, underground biomass, total biomass, leaf-stem ratio, and root-shoot ratio) were recorded. The results showed that the growth indices of ‘Reyan No. 5’ changed significantly under different root system separation methods. The aboveground, underground, and total biomass phenotypic indices showed a significant (P < 0.05) trend of no separation between roots < nylon mesh separation between roots and plastic film separation between roots. With no root separation, the plant height, the number of branches, aboveground, underground, and total biomass indices, and the leaf-stem ratio of ‘Reyan No. 5’ increased significantly (P < 0.05) when it was grown with ‘Reyan No. 24’. The number of branches and lateral roots, underground and total biomass, and leaf-stem ratio improved significantly (P < 0.05) when it was associated with ‘Reyan No. 21’. The aboveground and total biomass, and leaf-to-stem ratio of ‘Reyan No. 5’ was significantly (P < 0.05) reduced when it was grown with ‘Reyan No. 2’. Compared with the control group, the other growth indicators were not significantly different. The relative interaction index showed that the growth of ‘Reyan No. 5’ was promoted when it grew with ‘Reyan No. 21’ and ‘Reyan No. 24’, with ‘Reyan No. 24’ having a stronger effect. The growth of ‘Reyan No. 5’ was inhibited when grown with ‘Reyan No. 2’. These results suggest that ‘Reyan No. 5’ could identify the relationship between neighboring plants and itself through root exudates and then adopts a cooperative response strategy of controlling its growth with neighboring closely related plants.
- kin selection,
- root separation,
- root exudates,
- relative interaction index,
- morphological characteristics,
- biomass allocation,
- competitive strategy

FullText(HTML)

References (42)

References

[1]	DUDLEY S A, FILE A L. Kin recognition and competition in plants. Functional Ecology, 2013, 27(4): 898-906. doi: 10.1111/1365-2435.12121
[2]	李洁, 孙庚, 胡霞, 张洪轩, 刘琳, 吴宁. 植物的亲缘选择. 生态学报, 2014, 34(14): 3827-3838. LI J, SUN G, HU X, ZHANG H X, LIU L, WU N. Kin selection in plants. Acta Ecologica Sinica, 2014, 34(14): 3827-3838.
[3]	CREPY M A, CASAL J J. Photoreceptor-mediated kin recognition in plants. New Phytologist, 2015, 205(1): 329-338. doi: 10.1111/nph.13040
[4]	MURPHY G P, ACKER R V, RAJCAN I, SWANTON C J. Identity recognition in response to different levels of genetic relatedness in commercial soya bean. Royal Society Open Science, 2017, 4(1): 160879. doi: 10.1098/rsos.160879
[5]	BIEDRZYCKI M L, JILANY T A, DUDLEY S A, BAIS H P. Root exudates mediate kin recognition in plants. Communicative & Integrative Biology, 2010, 3(1): 28-35.
[6]	林威鹏, 彭莉, 肖桃艳, 蔡昆争. 植物亲缘识别的研究进展. 植物生态学报, 2015, 39(11): 1110-1121. doi: 10.17521/cjpe.2015.0108 LIN W P, PENG L, XIAO T Y, CAI K Z. Recent advance of kin recognition in plant. Chinese Journal of Plant Ecology, 2015, 39(11): 1110-1121. doi: 10.17521/cjpe.2015.0108
[7]	李洁, 孙庚, 吴宁, 王静, 胡霞. 一年生黑麦草的亲缘选择作用. 应用与环境生物学报, 2016, 22(6): 1063-1068. LI J, SUN G, WU N, WANG J, HU X. Kin selection in Lolium multiflorum L. Chinese Journal of Applied and Environmental Biology, 2016, 22(6): 1063-1068.
[8]	孔垂华. 植物种间和种内的化学作用. 应用生态学报, 2020, 31(7): 2141-2150. KONG C H. Inter-specific and intra-specific chemical interactions among plants. Chinese Journal of Applied Ecology, 2020, 31(7): 2141-2150.
[9]	FALIK O, DE KROON H, NOVOPLANSKY A. Physiologically-mediated self/nonself root discrimination in Trifolium repens has mixed effects on plant performance. Plant Signaling & Behavior, 2006, 1(3): 116-121.
[10]	CAFFARO M M, VIVANCO J M, BOTTO J, RUBIO G. Root architecture of Arabidopsis is affected by competition with neighbouring plants. Plant Growth Regulation, 2013, 70(2): 141-147. doi: 10.1007/s10725-013-9786-7
[11]	MERCER C A, EPPLEY S M. Kin and sex recognition in a dioecious grass. Plant Ecology, 2014, 215(8): 845-852. doi: 10.1007/s11258-014-0336-9
[12]	中国科学院中国植物志编辑委员会. 中国植物志第41卷. 北京: 科学出版社, 1995: 360-361. Chinese Plant Editorial Committee, Chinese Academy of Sciences. Flora Reipublicae Popularis Sinicae, Tomus 41. Beijing: Science Press, 1995: 360-361.
[13]	严琳玲, 张瑜, 白昌军. 13份柱花草品系生产性能比较. 草业科学, 2018, 35(4): 867-875. doi: 10.11829/j.issn.1001-0629.2017-0385 YAN L L, ZHANG Y, BAI C J. Comparative study on the production performance of 13 Stylosanthes germplasms. Pratacultural Science, 2018, 35(4): 867-875. doi: 10.11829/j.issn.1001-0629.2017-0385
[14]	刘国道, 白昌军, 何华玄, 王东劲, 周家锁. 热研5号柱花草选育研究. 草地学报, 2001, 9(1): 1-7. LIU G D, BAI C J, HE H X, WANG D J, ZHOU J S. The selection and utilization of Stylosanthes guianensis cv. Reyan No. 5. Acta Agrestia Ainica, 2001, 9(1): 1-7.
[15]	白昌军, 刘国道. 热研5号柱花草营养价值动态评价. 热带农业科学, 2002(2): 11-16, 23. doi: 10.3969/j.issn.1009-2196.2002.02.003 BAI C J, LIU G D. Dynamic evaluation of nutrition values of Stylosanthes guianensis cv. Reyan No. 5. Chinese Journal of Tropical Agriculture, 2002(2): 11-16, 23. doi: 10.3969/j.issn.1009-2196.2002.02.003
[16]	涂旭川, 刘国道, 白昌军, 张绪元, 朱新祥. 热研5号柱花草栽培技术初探. 中国农学通报, 2008, 24(11): 464-466. TU X C, LIU G D, BAI C J, ZHANG X Y, ZHU X X. Primary studied on planting technology of Stylosanthes Guianensis cv. Reyan No. 5. Chinese Agricultural Science Bulletin, 2008, 24(11): 464-466.
[17]	龙会英, 何华玄, 张德, 金杰, 史亮涛, 张明忠, 白昌军. 干热河谷退化山地柱花草品种(系)比较试验. 草业学报, 2011, 20(6): 230-236. doi: 10.11686/cyxb20110630 LONG H Y, HE H X, ZHANG D, JIN J, SHI L T, ZHANG M Z, BAI C J. Comparison of Stylosanthes guianensis in degraded mountain Yuanmou of the dry-hot Valley. Acta Prataculturae Sinica, 2011, 20(6): 230-236. doi: 10.11686/cyxb20110630
[18]	李满有, 马忠仁, 王斌, 杨雨琦, 沈笑天, 曹立娟, 李小云, 兰剑. 宁夏引黄灌区2个苜蓿品种不同行比混播模式研究. 草地学报, 2021, 29(4): 798-804. LI M Y, MA Z R, WANG B, YANG Y Q, SHEN X T, CAO L J, LI X Y, LAN J. Study on the mixed planting mode of two alfalfa varieties in different row ratios in the yellow river irrigation area in Ningxia. Acta Agrestia Sinica, 2021, 29(4): 798-804.
[19]	马建华, 赵紫华, 张蓉. 品种混播对苜蓿产量及主要害虫种群密度的调控. 草业科学, 2017, 34(12): 2521-2527. doi: 10.11829/j.issn.1001-0629.2017-0018 MA J H, ZHAO Z H, ZHANG R. Sowing a mixture of alfalfa modulates the population density of alfalfa pests. Pratacultural Science, 2017, 34(12): 2521-2527. doi: 10.11829/j.issn.1001-0629.2017-0018
[20]	郭世保, 黄丽丽, 康振生, 程晶晶, 陆宁海, 杨之为, 陈银潮. 小麦多品种混播控制条锈病的效果和机理研究. 中国农业科学, 2009, 42(10): 3485-3492. doi: 10.3864/j.issn.0578-1752.2009.10.0013 GUO S B, HUANG L L, KANG Z S, CHENG J J, LU N H, YANG Z W, CHEN Y C. Efficacy and mechanism of control of wheat stripe rust by diversifying cultivars in mix-planting. Scientia Agricultura Sinica, 2009, 42(10): 3485-3492. doi: 10.3864/j.issn.0578-1752.2009.10.0013
[21]	王俐媛, 王坚, 赵元辰, 许超, 李雪枫. 热研2号柱花草对不同亲缘植物根系分泌物的生物响应. 草业科学, 2022, 39(2): 1-9. doi: 10.11829/j.issn.1001-0629.2021-0331 WANG L Y, WANG J, ZHAO Y C, XU C, LI X F. Growth response of Stylosanthes guianensis Reyan No. 2 to root exudates from different relatives. Pratacultural Science, 2022, 39(2): 1-9. doi: 10.11829/j.issn.1001-0629.2021-0331
[22]	吴清莹, 林宇龙, 孙一航, 魏千皓, 刘婧婷, 李雪峰, 崔国文. 根系分泌物对植物生长和土壤养分吸收的影响研究进展. 中国草地学报, 2021, 43(11): 97-104. WU Q Y, LIN Y L, SUN Y H, WEI Q H, LIU J T, LI X F, CUI G W. Research progress on effect of root exudates on plant growth and soil nutrient uptake. Chinese Journal of Grassland, 2021, 43(11): 97-104.
[23]	杨富玲, 石杨, 李斌, 杜志烨, 汪梦婷, 廖恒毅, 陈稷, 黄进. 植物根系分泌物在污染及沙化土壤修复中的应用现状与前景. 应用生态学报, 2021, 32(7): 2623-2632. YANG F L, SHI Y, LI B, DU Z Y, WANG M T, LIAO H Y, CHEN J, HUANG J. Status and prospects of the application of root exudates in the restoration of polluted or desertated soil. Chinese Journal of Applied Ecology, 2021, 32(7): 2623-2632.
[24]	蒋侯明, 何朝族, 李居正, 林坚毅. 热带优良豆科牧草: 热研2号柱花草的选育及推广. 热带作物研究, 1992(1): 62-66. JIANG H M, HE C Z, LI J Z, LIN J Y. Breeding and promotion of excellent tropical leguminous forages: Stylosanthes guianensis cv. Reyan No. 2. Chinese Journal of Tropical Agriculture, 1992(1): 62-66.
[25]	刘国道. 热研21号圭亚那柱花草. (2011-05-16) [2022-01-15]. https://kns.cnki.net/kcms2/article/abstract?v=3q6cCi6hV7MyBf2cYy8SywPrOgGZO3X6R21eE58CKacxJ7u-XAxWdcUvsqy7vs1_ZbA_u6QoF1wKHVMFyhhK5HRsjJCMO6b8FPtDBsKkZQBbdBXIV90Udg==&uniplatform=NZKPT&language=CHSmod=view&aid=667625. LIU G D. Stylosanthes guianensis cv. Reyan No. 21. Tropical Crop Germplasm Research Institute. (2011-05-16) [2022-01-15]. https://kns.cnki.net/kcms2/article/abstract?v=3q6cCi6hV7MyBf2cYy8SywPrOgGZO3X6R21eE58CKacxJ7u-XAxWdcUvsqy7vs1_ZbA_u6QoF1wKHVMFyhhK5HRsjJCMO6b8FPtDBsKkZQBbdBXIV90Udg==&uniplatform=NZKPT&language=CHS
[26]	中国热带农业科学院热带作物品种资源研究所. 中国热科院品资所四个新品种通过国家牧草品种审定. (2020-01-04) [2021-02-28]. https://www.catas.cn/contents/5/144401.html Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences. Four new varieties of Centrosemapubescens benth, Stylosanthes guianensis cv. Reyan No. 24, Canna edulis and Coix lacryma-jobi have passed the examination and approval of national forage varieties.(2020-01-04) [2021-02-28]. https://www.catas.cn/contents/5/144401.html
[27]	汪雪, 刘晓静, 赵雅姣, 王静. 根系分隔方式下紫花苜蓿/燕麦间作氮素利用及种间互馈特征研究. 草业学报, 2021, 30(8): 73-85. doi: 10.11686/cyxb2021037 WANG X, LIU X J, ZHAO Y J, WANG J. Nitrogen utilization and interspecific feedback characteristics of intercropped alfalfa/oat with different root barriers. Acta Prataculturae Sinica, 2021, 30(8): 73-85. doi: 10.11686/cyxb2021037
[28]	王亚露, 赵建宁, 许彦骁, 张乐, 杨殿林, 李洁. 种间竞争对香蒲与芦苇生长的影响. 生态学报, 2022(7): 1-8. WANG Y L, ZHAO J N, XU Y X, ZHANG L, YANG D L, LI J. Effects of interspecific competition on the growth of Typha domingensis and Phragmites australis. Acta Ecologica Sinica, 2022(7): 1-8.
[29]	CHIUFFO M C, MACDOUGALL A S, HIERRO J L. Native and non-native ruderals experience similar plant-soil feedbacks and neighbor effects in a system where they coexist. Oecologia, 2015, 179(3): 843-852. doi: 10.1007/s00442-015-3399-y
[30]	李洁, 庞蕊, 徐兴良, 梁涛. 高粱对不同身份邻居的形态学与生理学响应. 应用与环境生物学报, 2017, 23(5): 800-805. LI J, PANG R, XU X L, LIANG T. Morphological and physiological responses for neighbor identity in Sorghum vulgare. Chinese Journal of Applied & Environmental Biology, 2017, 23(5): 800-805.
[31]	蒋亚君, 申晴, 丁西朋, 严琳玲, 刘国道, 白昌军. 柱花草种质资源表型性状的多样性分析. 草业科学, 2017, 34(5): 1032-1041. doi: 10.11829/j.issn.1001-0629.2016-0588 JIANG Y J, SHEN Q, DING X P, YAN L L, LIU G D, BAI C J. Phenotypic diversity analysis of Stylosanthes germplasms. Pratacultural Science, 2017, 34(5): 1032-1041. doi: 10.11829/j.issn.1001-0629.2016-0588
[32]	白昌军, 刘国道, 柱花草良种繁育技术与管理. 南京: 江苏科学技术出版社, 2013: 37-38. BAI C J, LIU G D. Technique and Management of Fine Species Breeding of Stylosanthes. Nanjing: Jiangsu Science and Technology Press, 2013: 37-38.
[33]	BHATT M V, KHANDELWAL A, DUDLEY S A. Kin recognition, not competitive interactions, predicts root allocation in young Cakile edentula seedling pairs. New Phytologist, 2011, 189(4): 1135-1142. doi: 10.1111/j.1469-8137.2010.03548.x
[34]	GERSANI M, BROWN J S, O'BRIEN E E, MAINA G M, ABRAMSKY Z. Tragedy of the commons as a result of root competition. Journal of Ecology, 2001, 89(4): 660-669. doi: 10.1046/j.0022-0477.2001.00609.x
[35]	DONOHUE K. The influence of neighbor relatedness on multilevel selection in the Great Lakes sea rocket. American Naturalist, 2003, 162(1): 77-92. doi: 10.1086/375299
[36]	杨雪芳. 化感水稻对邻近植物的生物化学响应及其化感物质衍生物的抑草机制. 北京: 中国农业大学博士学位论文, 2017. YANG X F. Biological and chemical responses of allelopathic rice to neighboring plants and weed-suppressive mechanisms of rice allelochemical derivatives. PhD Thesis. Beijing: China Agricultural University, 2017.
[37]	MURPHY G P, DUDLEY S A. Kin recognition: Competition and cooperation in Impatiens (Balsaminaceae). American Journal of Botany, 2009, 96(11): 1990-1996. doi: 10.3732/ajb.0900006
[38]	TILL-BOTTRAUD I, DE VILLEMEREUIL P. Kin recognition or phenotype matching. New Phytologist, 2016, 209(1): 13-14. doi: 10.1111/nph.13554
[39]	BARTELHEIMER M, GOWING D, SILVERTOWN J. Explaining hydrological niches: The decisive role of below-ground competition in two closely related Senecio species. Journal of Ecology, 2010, 98(1): 126-136. doi: 10.1111/j.1365-2745.2009.01598.x
[40]	FANG S Q, CLARK R T, ZHENG Y, IYER-PASCUZZI A S, WEITZ J S, KOCHIAN L V, EDELSBRUNNER H, LIAO H, BENFEY P N. Genotypic recognition and spatial responses by rice roots. Proceedings of the National Academy of Sciences of the United States, 2013, 110(7): 2670-2675. doi: 10.1073/pnas.1222821110
[41]	董楠, 唐明明, 崔文倩, 岳梦瑶, 刘洁, 黄玉杰. 不同根系分隔方式对栗和茶幼苗生长的影响. 植物生态学报, 2022, 46(1): 62-73. doi: 10.17521/cjpe.2021.0255 DONG N, TANG M M, CUI W Q, YUE M Y, LIU J, HUANG Y J. Growth of chestnut and tea seedlings under different root partitioning patterns. Chinese Journal of Plant Ecology, 2022, 46(1): 62-73. doi: 10.17521/cjpe.2021.0255
[42]	DE WIT M, KEGGE W, EVERS J B, EIJK MHV, GANKEMA P, VOESENEK LACJ, PIERIK R. Plant neighbor detection through touching leaf tips precedes phytochrome signals. Proceedings of the National Academy of Sciences of the United States of America, 2012, 109(36): 14705-14710. doi: 10.1073/pnas.1205437109

Cited By

Get Citation

PDF

XML

Article views (881) PDF downloads (12)

Turn off MathJax

Article Contents

Abstract

References

Effect of different root separation methods on kin recognition and selection of Stylosanthes guianensis ‘Reyan No. 5’

Abstract

References

Catalog

Export File

Citation

Format

Content