Physiological and fluorescence characteristics of Anemone silvestris in response to high temperature stress

Using wild Anemone silvestris seedlings as experimental material, we studied the effects of high temperature stress on the leaf physiology and fluorescence characteristics of A. silvestris plants to reveal its heat resistance mechanism and provide theoretical guidance for quality introduction and cultivation. The results showed that with the increase in the duration of high temperature stress treatment, the semilethal temperature of A. silvestris gradually decreased. The semilethal temperature was 50.83 ℃ under 12 h treatment time and 36.03 ℃ under 72 h treatment. At the same treatment time, the relative electrical conductivity, malondialdehyde content, carotenoid content, and maximum quantum yield of nonphotochemical burst (φDo) of A. silvestris leaves increased with increasing temperature compared with 25 ℃, while the potential activity (F_v/F_o), maximum photochemical quantum yield (F_v/F_m), and maximum photochemical efficiency (φPo) decreased gradually. When the plant cells are damaged by heat, the plant adapts to the high temperature environment by adjusting its own protection mechanism, improving its antioxidant capacity, and dissipating the unavailable light energy into the air in the form of heat energy. Therefore, we can conclude that wild A. silvestris has certain heat resistance and can be utilized as a potential garden plant resource in northern areas.