Isolation and identification of dominant lactic acid bacteria from Urtica cannabina silage

We isolated and identified dominant lactic acid bacteria in Urtica cannabina silage and provided a theoretical basis for improving the quality of U. cannabina silage. Through traditional microbial and molecular biological identification, 11 strains of dominant lactic acid bacteria were isolated from U. cannabina silage. Strain QM7 was identified as Lactococcus garvieae, QM16 was identified as Enterococcus faecalis, QM35 was identified as Enterococcus gallinarum, QM13 and QM32 were identified as Enterococcus casseliflavus, QM6, QM8, and QM10 were identified as Weissella paramesenteroides, and QM9, QM11, and QM12 were identified as Pediococcus pentosaceus. In addition, the isolated lactic acid bacteria were cultured at 4～45 ℃ and in a medium containing 3.0% and 6.5% NaCl. None of the strains grew at pH 3.0, and growth or weak growth was observed at pH 3.5～9. The pH values of strains QM7 (L. garvieae) and QM11 (P. pentosaceus) decreased to 3.82 and 3.78 after 24 h of culture, and the OD₆₀₀ values ​​were 2.12 and 2.06, exhibiting the strongest acid production capacity and growth rate. These strains may be useful as fungal agents for regulating nettle silage in later stages.