Identification of Polygonum viviparum endophytic bacteria Z17 and its capacity to antagonistic towards pathogenic fungi
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Abstract
Polygonum viviparum is the dominant species of alpine meadow community. A experiment was conducted to estimate the capacity of P. viviparum endophytic bacteria Z17 to antagonistic towards pathogenic fungi by PDA plates and to determine the taxonomic status of Z17 by morphological and molecular biology methods.The results of this study showed that Z17 inhibited Bipolaria maydis, Rhizoctonia solani, Sclerotinia sclerotiorum, Fusarium oxysporum f. Niveum, Alternaria solani, Botrytis cinerea, Bipolaris sorokiniana, indicating that Z17 had a wide antibacterial spectrum. Z17 was rod shape with a size of (1.4-3.6) m (0.4-0.6) m, Grampositive, and produced spore in the center, and it was same morphological characteristics with Bacillus sp. Similarity between 16S rDNA gene sequence of Z17 and Bacillus mojavensis model strain BCRC 17531 reached 99.79%, and the genetic distance between Z17 and BCRC 17531 in the phylogenetic tree was less than 0.000 5. These results suggested that Z17 could be identified as Bacillus mojavensis and had the potential to be explored as Biopesticides.
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