In vitro culture of floral organ of Lilium cernum

The petal, ovary and receptacle of Lilium cernum were used as explants for in vitro culture. The results showed that the rates of callus induction and differentiation of three kinds of floral organ explants were different. The petals had the highest callus induction and differentiation rates, with 57.65% and 81.12%, respectively. The order from easy to difficult of callus induction was petalreceptacleovary. The optimum medium for petal explants callus induction and differentiation was MS+6BA 2.0 mgL-1+NAA 0.5 mgL-1. The optimum medium for ovary explants callus induction and differentiation was MS+6BA 1.0 mgL-1 +NAA 0.5 mgL-1. The optimum medium for receptqacle explants callus induction and differentiation was MS+6BA 1.0 mgL-1 +NAA 0.2 mgL-1. The optimum medium for rooting was 1/2 MS+IBA 1.0 mgL-1 +NAA 0.3 mgL-1+AC 0.5 gL-1.