Effect of detergent concentrations on root tip cells teratogenesis of pea

the micronucleus test was used to determine the effects of detergent concentrations on root tip cells teratogenesis of pea by measuring the cytotoxicity and the genotoxicity. In this study, the dilution detergent concentrations with 200 volume (detergent/distilled water), 100 volume, 10 volume and 5 volume, respectively, was added into pea root tip and root point cell mitosis index, the micronucleus rate and chromosomic aberration rate were investigated and made micrography at 24 h and 48 h. The results of this study showed that the root tip cell mitosis index obviously decreased and had the obvious dosagetime effectiveness, and the micronucleus rate and the chromosomic aberration rate increased in low concentrations, but they decreased in high concentrations and they did not have the obvious dosagetime effect along with the increase of detergent diluted concentration and with the lengthens of treatment time. The cell chromosome bridge, micronucleus and many kinds of chromosomic aberration type were caused with different concentrations detergent, and the root tip cell chromosome damage was created as well. This study suggested that the different concentrations detergent had some certain heredity toxicity to the pea root tip cell.