Construction of plant expression vector containing Arabidopsis transcription factor CBF4

CBF4 gene was obtained by enzyme cutting with both SalI and EcoRI, and inserted into Bluescript M13（SK）, thus the intermediate vector SKCBF4 was formed. Then, SKCBF4 and HpBPC26CBF4 were double digestion using BamHI and KpnI，respectively, and connected them using T4DNA. Finally，the plant expression vector of HpBPCCBF4 has been constructed. The results of PCR and enzyme digestion showed the plant expression vectors were correct and can be use in the genetic transformation of monocotyledon.